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IHCPlus™ CCL20 / MIP‑3‑Alpha Antibody Monoclonal ELISA,IHC,WB LS‑B7409

Catalog Number Size Price
LS-B7409-500 500 µg (1 mg/ml) $425 
Anti-CCL20 antibody IHC of human spleen, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Human MIP-3a (CCL20) Western Blot Unreduced
Anti-Human MIP-3a (CCL20) Sandwich ELISA
Anti-CCL20 antibody IHC of human spleen, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Human MIP-3a (CCL20) Western Blot Unreduced
Anti-Human MIP-3a (CCL20) Sandwich ELISA
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IHCPlus™ CCL20 / MIP‑3‑Alpha Antibody Monoclonal ELISA,IHC,WB LS‑B7409

Note: This antibody replaces LS-C104419
Antibody:
Mouse Monoclonal to Human CCL20 / MIP-3-Alpha
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B7409-500
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human CCL20 / MIP-3-Alpha
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
MIP-3-Alpha antibody LS-B7409 is an unconjugated mouse monoclonal antibody to human MIP-3-Alpha (CCL20 ). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human CCL20 / MIP-3-Alpha
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 0.4 µg/ml)
  • ELISA (2 - 4 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
E.coli derived recombinant Human MIP-3alpha.
Specificity
Human MIP-3alpha
Usage
Immunohistochemistry: LS-B7409 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant Human MIP-3alpha when used with biotinylated antigen affinity purified anti-Human MIP-3alpha (LS-C104612) as the detection antibody at a concentration of approximately 1-2 ug/ml. Western Blot: To detect Human MIP-3alpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3alpha is 0.25-0.50 ng/lane, under non-reducing conditions.
Presentation
Lyophilized from PBS
Reconstitution
500 ul sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL20 / MIP-3-Alpha
P78556 NM_004591 NP_004582.1

Validation

Anti-CCL20 antibody IHC of human spleen, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC Validated
Anti-CCL20 antibody IHC of human spleen, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CCL20 antibody IHC of human spleen, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Publications (1)

Immunotopographical Differences of Human Skin. Béke G, Dajnoki Z, Kapitány A, Gáspár K, Medgyesi B, Póliska S, Hendrik Z, Péter Z, Törocsik D, Bíró T, Szegedi A. Frontiers in immunology. 2018 March;9:424. (Human)

Reviews (0)

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Requested From: United States
Date Requested: 9/19/2019