Human CASZ1

Rabbit

Human, Chimpanzee, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)

Human, Chimpanzee, Mouse, Rat, Bovine (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to region near the amino terminal end of human Cas5 protein.

A BLAST analysis was used to suggest cross reactivity with Cas5 from human, mouse, chimpanzee, bovine, and rat based on 100% homology with the immunizing sequence. Expect cross reactivity with multiple isoforms of this protein. Cross reactivity with Cas5 from other sources has not been determined.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1:500 - 1:2000)
• ELISA (1:10000 - 1:50000)

Immunohistochemistry: LS-B2838 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2838 was determined to be 5 ug/ml. Western Blot: A band approximately 125 kD in size corresponding to hCas5 protein is expected in the appropriate cell lysate or extract.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C or below prior to opening. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Dilute only prior to immediate use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q86V15 NCBI: NM_017766 NP_060236.3