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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3473-50 | 50 µl | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human CALR3 Antibody (clone 1G6A7, IHC, IF, WB) LS‑B3473
IHC‑plus™ Monoclonal Mouse anti‑Human CALR3 Antibody (clone 1G6A7, IHC, IF, WB) LS‑B3473
Note: This antibody replaces LS-C41528, LS-C171397
Antibody:
CALR3 Mouse anti-Human Monoclonal (1G6A7) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CALR3 Mouse anti-Human Monoclonal (1G6A7) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CALR3 antibody LS-B3473 is an unconjugated mouse monoclonal antibody to human CALR3. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CALR3
Synonyms
CALR3 | Calreticulin 3 | Calreticulin-2 | Calreticulin-3 | Calsperin | CRT2 | CT93 | CMH19 | Cancer/testis antigen 93
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Clone
1G6A7
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to aa (EEEDVPGQAKDELC) of human Calreticulin, conjugated to KLH.\
Specificity
Human, Mouse
Applications
- IHC
- IHC - Paraffin (1:200)
- Immunofluorescence (1:200 - 1:1000)
- Western blot (1:100 - 1:500)
- ELISA (1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3473 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3473 was determined to be 1:200.
Presentation
Ascitic fluid, 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Anti-Calreticulin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-Calreticulin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-Calreticulin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-Calreticulin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
See More About...
LSBio Ratings
IHC-plus™ CALR3 Antibody (clone 1G6A7) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B3473 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human, Monkey, Bat
Applications:
IHC - Paraffin
Reactivity:
Monkey
Range:
15.63-1000 pg/ml
Reactivity:
Human
Range:
40-10000 pg/ml
Reactivity:
Human
Range:
0.78-50 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
