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Catalog Number Size Price
LS-B1778-50 50 µg (1 mg/ml) $440 
C5AR2 / GPR77 / C5L2 Antibody - Anti-C5L2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
C5AR2 / GPR77 / C5L2 Antibody - Western blot ofugPR77 in human testis?cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at 0.5 ug/ml.
C5AR2 / GPR77 / C5L2 Antibody - Anti-C5L2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
C5AR2 / GPR77 / C5L2 Antibody - Western blot ofugPR77 in human testis?cell lysate in the 1) absence and 2) presence of immunizing peptide using antibody at 0.5 ug/ml.
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IHC‑plus™ Polyclonal Rabbit anti‑Human C5AR2 / GPR77 / C5L2 Antibody (aa1‑50, IHC, WB) LS‑B1778

IHC‑plus™ Polyclonal Rabbit anti‑Human C5AR2 / GPR77 / C5L2 Antibody (aa1‑50, IHC, WB) LS‑B1778

Note: This antibody replaces LS-C47287, LS-C66954
Antibody:
C5AR2 / GPR77 / C5L2 Rabbit anti-Human Polyclonal (aa1-50) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B1778-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
C5AR2 / GPR77 / C5L2 Rabbit anti-Human Polyclonal (aa1-50) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
C5L2 antibody LS-B1778 is an unconjugated rabbit polyclonal antibody to human C5L2 (C5AR2 / GPR77) (aa1-50). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human C5AR2 / GPR77 / C5L2
Synonyms
C5AR2 | G-protein coupled receptor 77 | GPF77 | GPR77 | C5L2 | G protein-coupled receptor 77
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A portion of amino acids 1-50 of human GPR77.
Epitope
aa1-50
Specificity
The amino acid sequence used as immunogen is 100% homologous in human, and chimp (isoforms 1, 2, 3, and 4), 88% homologous in rhesus monkey (isoforms 1, 2, 3), and 76% homologous in bovine (isoforms 1 and 2).
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B1778 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1778 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About C5AR2 / GPR77 / C5L2
Q9P296 NM_018485 NP_060955.1

Validation

C5AR2 / GPR77 / C5L2 Antibody - Anti-C5L2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-C5L2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-C5L2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ C5AR2 / GPR77 / C5L2 Antibody (aa1-50) for IHC, WB/Western LS-B1778 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/21/2021