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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B5229-0.05 0.05 mg (1 mg/ml) $425 
C5AR1 / CD88 / C5a Receptor Antibody - Anti-C5AR1 / CD88 antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHCPlus™ Monoclonal Rat anti‑Mouse C5AR1 / CD88 / C5a Receptor Antibody (clone 10/92, IHC, IF) LS‑B5229

IHCPlus™ Monoclonal Rat anti‑Mouse C5AR1 / CD88 / C5a Receptor Antibody (clone 10/92, IHC, IF) LS‑B5229

Note: This antibody replaces LS-C44868, LS-C57964, LS-C44866
Antibody:
Rat Monoclonal to Mouse C5AR1 / CD88 / C5a Receptor
Application:
IHC, IHC-P, IHC-Fr, IF, Flo
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B5229-0.05
0.05 mg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rat Monoclonal to Mouse C5AR1 / CD88 / C5a Receptor
Application:
IHC, IHC-P, IHC-Fr, IF, Flo
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
C5a Receptor antibody LS-B5229 is an unconjugated rat monoclonal antibody to mouse C5a Receptor (C5AR1 / CD88). Validated for Flow, IF and IHC.
Target
Mouse C5AR1 / CD88 / C5a Receptor
Synonyms
C5AR1 | C5A | C5a-R | C5a ligand | C5a receptor | CD88 | C5a anaphylatoxin receptor | C5AR | C5R1 | CD88 antigen
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
10/92
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • Immunofluorescence
  • Flow Cytometry (1:50 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
RBL-2H3 transfected cells expressing murine C5aR
Specificity
Recognizes murine CD88, a 45kD G-protein coupled cell surface receptor, which is otherwise known as C5aR. The CD88 molecule functions as a receptor for the complement component C5a, which is a potent proinflammatory molecule and a chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes. Clone 10/92 does not block the binding of the C5a to murine CD88.
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About C5AR1 / CD88 / C5a Receptor
P21730 NM_001736 NP_001727.1

Validation

C5AR1 / CD88 / C5a Receptor Antibody - Anti-C5AR1 / CD88 antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-C5AR1 / CD88 antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-C5AR1 / CD88 antibody IHC of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ C5AR1 / CD88 / C5a Receptor Antibody (clone 10/92) for IHC, IF/Immunofluorescence, Flow LS-B5229 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 7/12/2020