Human BRCC3 / BRCC36

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

AT3B1

Unconjugated

Protein G purified

Unmodified

Recombinant BRCC36 (1-316aa) purified from E. coli

BRCC36 (1-316aa)

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1:1000 - 1:2000)
• ELISA

Immunohistochemistry: LS-B3588 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3588 was determined to be 5 ug/ml. Western Blot: Cell lysates of HeLa(20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

PBS, pH 7.4, 0.1% Sodium Azide

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P46736 NCBI: NM_024332 NP_077308.1