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IHCPlus™ BRAP2 Antibody aa67‑79 Polyclonal ELISA,IHC LS‑B3402

Catalog Number Size Price
LS-B3402-50 50 µg (1 mg/ml) $425 
Anti-BRAP antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHCPlus™ BRAP2 Antibody aa67‑79 Polyclonal ELISA,IHC LS‑B3402

Note: This antibody replaces LS-C3217
Antibody:
Rabbit Polyclonal to Human BRAP2
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3402-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BRAP2
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
BRAP2 antibody LS-B3402 is an unconjugated rabbit polyclonal antibody to BRAP2 from human and monkey. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human BRAP2
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Mouse, Dog, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ELISA (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human BRAP. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mouse, Elephant, Panda, Dog, Horse (92%); Hamster, Rabbit, Pig (85%).
Epitope
aa67-79
Specificity
KLH conjugated synthetic peptide comprising amino acids 27 - 39 [AAGEMSDEEIKKT] of the human BRCA1 associated protein (BRAP) [N-terminus] protein.
Usage
Immunohistochemistry: LS-B3402 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3402 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BRAP2
Q7Z569 NM_006768 NP_006759.3

Validation

Anti-BRAP antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC Validated
Anti-BRAP antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-BRAP antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

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Featured Products

Immunohistochemistry image at a dilution of 1:300 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Product
Species: Human
Applications: Immunofluorescence, Flow Cytometry, ELISA
Product
Species: Human
Applications: Immunofluorescence, Flow Cytometry, ELISA
IHC testing of FFPE human breast carcinoma tissue with BRCA1 antibody (clone BRCA1/1398). HIER: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min.
Species: Human
Applications: IHC - Paraffin, Immunofluorescence, Flow Cytometry
Reactivity: Human
Range: 31.25-2000 pg/ml

Requested From: United States
Date Requested: 9/24/2019