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Catalog Number Size Price
LS-A1512-50 50 µg (1 mg/ml) $460 
BOB / GPR15 Antibody - Anti-GPR15 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC‑plus™ Polyclonal Rabbit anti‑Human BOB / GPR15 Antibody (C‑Terminus, IHC) LS‑A1512

IHC‑plus™ Polyclonal Rabbit anti‑Human BOB / GPR15 Antibody (C‑Terminus, IHC) LS‑A1512

Antibody:
BOB / GPR15 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$460
LS-A1512-50

Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
BOB / GPR15 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
GPR15 antibody LS-A1512 is an unconjugated rabbit polyclonal antibody to human GPR15 (BOB) (C-Terminus). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human BOB / GPR15
Synonyms
GPR15 | Brother of Bonzo | G protein-coupled receptor 15 | G-protein coupled receptor 15 | BOB | Gpr-15
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 19 amino acid peptide from C-Terminus cytoplasmic domain of human GPR15. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%); Monkey (95%); Marmoset, Mouse, Bat, Bovine, Elephant, Panda, Rabbit (89%); Dog, Hamster, Horse, Pig (84%).
Epitope
C-Terminus
Specificity
Human GPR15. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BOB / GPR15
P49685 NM_005290 NP_005281.1

Validation

BOB / GPR15 Antibody - Anti-GPR15 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR15 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR15 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

IHC-plus™ BOB / GPR15 Antibody (C-Terminus) for IHC, ELISA LS-A1512 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/28/2021