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IHCPlus™ BECN1 / Beclin‑1 Antibody Polyclonal IHC,WB LS‑B510

Catalog Number Size Price
LS-B510-50 50 µl (1 mg/ml) $425 
Anti-BECN1 / Beclin-1 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHCPlus™ BECN1 / Beclin‑1 Antibody Polyclonal IHC,WB LS‑B510

Note: This antibody replaces LS-C2536
Antibody:
Rabbit Polyclonal to Human BECN1 / Beclin-1
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B510-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BECN1 / Beclin-1
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Beclin-1 antibody LS-B510 is an unconjugated rabbit polyclonal antibody to Beclin-1 (BECN1 ) from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BECN1 / Beclin-1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1.25 µg/ml)
  • Western blot (1:2000 - 1:5000)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide to human Beclin 1, containing residues within 1-100. This sequence has 94% identity with the mouse sequence and 88% identity with the rat sequence
Specificity
Beclin 1.
Usage
Immunohistochemistry: LS-B510 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B510 was determined to be 1.25 ug/ml.
Presentation
TBS, 5 mg/ml BSA, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: -70°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BECN1 / Beclin-1
Q14457 NM_003766 NP_003757.1

Validation

Anti-BECN1 / Beclin-1 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-BECN1 / Beclin-1 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-BECN1 / Beclin-1 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Requested From: United States
Date Requested: 9/17/2019