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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B3202-0.05 0.05 mg (1 mg/ml) $425 
BECN1 / Beclin-1 Antibody - Anti-BECN1 / Beclin-1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
BECN1 / Beclin-1 Antibody - Anti-BECN1 / Beclin-1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
BECN1 / Beclin-1 Antibody - Anti-BECN1 / Beclin-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
BECN1 / Beclin-1 Antibody - Immunocytochemistry staining of A431 cells using Beclin-1 antibody at 1 µg/ml.
BECN1 / Beclin-1 Antibody - Immunofluorescence of Beclin-1 in A431 cells with Beclin-1 antibody at 2 ug/ml.
BECN1 / Beclin-1 Antibody - Western blot of Beclin-1 in A431 cell lysate with Beclin-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
BECN1 / Beclin-1 Antibody - Anti-BECN1 / Beclin-1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
BECN1 / Beclin-1 Antibody - Anti-BECN1 / Beclin-1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
BECN1 / Beclin-1 Antibody - Anti-BECN1 / Beclin-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
BECN1 / Beclin-1 Antibody - Immunocytochemistry staining of A431 cells using Beclin-1 antibody at 1 µg/ml.
BECN1 / Beclin-1 Antibody - Immunofluorescence of Beclin-1 in A431 cells with Beclin-1 antibody at 2 ug/ml.
BECN1 / Beclin-1 Antibody - Western blot of Beclin-1 in A431 cell lysate with Beclin-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
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IHCPlus™ Polyclonal BECN1 / Beclin‑1 Antibody (IHC, IF, WB) LS‑B3202

IHCPlus™ Polyclonal BECN1 / Beclin‑1 Antibody (IHC, IF, WB) LS‑B3202

Note: This antibody replaces LS-C19536, LS-C127025
Antibody:
Rabbit Polyclonal to Human BECN1 / Beclin-1
Application:
IHC, IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3202-0.05
0.05 mg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human BECN1 / Beclin-1
Application:
IHC, IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Beclin-1 antibody LS-B3202 is an unconjugated rabbit polyclonal antibody to Beclin-1 (BECN1) from human and mouse. Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BECN1 / Beclin-1
Synonyms
BECN1 | ATG6 | Beclin 1 | Beclin 1, autophagy related | Beclin1 | GT197 | Protein GT197 | VPS30 | Beclin-1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (0.5 - 1 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A 16 AA synthetic peptide near the C-Terminus of human Beclin-1. The immunogen is located within the last 50 AA of Beclin-1.
Blocking Peptide
LS-E30177 - Liquid - 0.05 mg 200 µg/ml - $145.00
Immunizing peptide used to generate LS-B3202. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
Beclin-1 antibody can be used for the detection of Beclin-1 by Western blot at 0.5 - 2 ug/mL. Antibody can also be used for immunocytochemistry starting at 1 ug/mL. For immunofluorescence start at 2 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BECN1 / Beclin-1
Q14457 NM_003766 NP_003757.1

Validation

BECN1 / Beclin-1 Antibody - Anti-BECN1 / Beclin-1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BECN1 / Beclin-1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BECN1 / Beclin-1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

BECN1 / Beclin-1 Antibody - Anti-BECN1 / Beclin-1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BECN1 / Beclin-1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BECN1 / Beclin-1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

BECN1 / Beclin-1 Antibody - Anti-BECN1 / Beclin-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BECN1 / Beclin-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BECN1 / Beclin-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ BECN1 / Beclin-1 Antibody for IHC, ICC, IF/Immunofluorescence, WB/Western, ELISA LS-B3202 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

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Requested From: United States
Date Requested: 4/5/2020