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IHCPlus™ BECN1 / Beclin‑1 Antibody Polyclonal ELISA,ICC,IF,IHC,WB LS‑B3202

Catalog Number Size Price
LS-B3202-0.05 0.05 mg (1 mg/ml) $425 
Anti-BECN1 / Beclin-1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BECN1 / Beclin-1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BECN1 / Beclin-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunocytochemistry staining of A431 cells using Beclin-1 antibody at 1 µg/ml.
Immunofluorescence of Beclin-1 in A431 cells with Beclin-1 antibody at 2 ug/ml.
Western blot of Beclin-1 in A431 cell lysate with Beclin-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Anti-BECN1 / Beclin-1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BECN1 / Beclin-1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BECN1 / Beclin-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunocytochemistry staining of A431 cells using Beclin-1 antibody at 1 µg/ml.
Immunofluorescence of Beclin-1 in A431 cells with Beclin-1 antibody at 2 ug/ml.
Western blot of Beclin-1 in A431 cell lysate with Beclin-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
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IHCPlus™ BECN1 / Beclin‑1 Antibody Polyclonal ELISA,ICC,IF,IHC,WB LS‑B3202

Note: This antibody replaces LS-C19536, LS-C127025
Antibody:
Rabbit Polyclonal to Human BECN1 / Beclin-1
Application:
IHC, IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3202-0.05
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BECN1 / Beclin-1
Application:
IHC, IHC-P, ICC, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Beclin-1 antibody LS-B3202 is an unconjugated rabbit polyclonal antibody to Beclin-1 (BECN1 ) from human and mouse. Validated for ELISA, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BECN1 / Beclin-1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • ICC
  • Immunofluorescence
  • Western blot (0.5 - 1 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
a 16 amino acid synthetic peptide near the C-terminus of human Beclin-1. The immunogen is located within the last 50 amino acids of Beclin-1.
Blocking Peptide
LS-E30177 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B3202. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
Beclin-1 antibody can be used for the detection of Beclin-1 by Western blot at 0.5 - 2 ug/mL. Antibody can also be used for immunocytochemistry starting at 1 ug/mL. For immunofluorescence start at 2 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BECN1 / Beclin-1
Q14457 NM_003766 NP_003757.1

Validation

Anti-BECN1 / Beclin-1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-BECN1 / Beclin-1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BECN1 / Beclin-1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-BECN1 / Beclin-1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-BECN1 / Beclin-1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BECN1 / Beclin-1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-BECN1 / Beclin-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-BECN1 / Beclin-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BECN1 / Beclin-1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Staining of Beclin 1 in mouse lung.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Anti-BECN1 / Beclin-1 antibody IHC of human adrenal medulla. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 25 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Range: 0.156-10 ng/ml

Requested From: United States
Date Requested: 9/17/2019