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IHCPlus™ BACH2 Antibody C‑Terminus Polyclonal IHC,WB LS‑B3533

Catalog Number Size Price
LS-B3533-100 100 µl (0.5 mg/ml) $485 
Anti-BACH2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-BACH2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
BACH2 antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-BACH2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-BACH2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
BACH2 antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ BACH2 Antibody C‑Terminus Polyclonal IHC,WB LS‑B3533

Note: This antibody replaces LS-C101773
Antibody:
Rabbit Polyclonal to Human BACH2
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B3533-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BACH2
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Rabbit
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
BACH2 antibody LS-B3533 is an unconjugated rabbit polyclonal antibody to BACH2 from human, monkey and rabbit. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human BACH2
Host
Rabbit
Reactivity
Human, Monkey, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 - 5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from C-Terminus of human BACH2 (Q9BYV9, NP_068585). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset (100%); Gibbon, Mouse, Rat, Horse, Guinea pig (92%); Galago, Elephant, Rabbit (85%).
Blocking Peptide
LS-E14293 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3533. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human BACH2
Usage
Immunohistochemistry: LS-B3533 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3533 was determined to be 2.5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BACH2
Q9BYV9 NM_021813 NP_068585.1

Validation

Anti-BACH2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-BACH2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-BACH2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Anti-BACH2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-BACH2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-BACH2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Publications (1)

Increase of T and B cells and altered BACH2 expression patterns in bone marrow trephines of imatinib-treated patients with chronic myelogenous leukaemia. Von Laffert M, Hänel M, Dietel M, Anagnostopoulos I, Jöhrens K. Oncology letters. 2016 October;12:2421-2428. (Human)

Reviews (0)

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Western Blot of Rabbit anti-Bach2 antibody. Lane 1: 293T cell lysates overexpressing Bach2-Flag. Lane 2: 293T cell lysates. Load: 20 ug per lane. Primary antibody: Bach-2 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: rabbit HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 91.7 kDa, ~130 kDa for Bach2. Other band(s): none.
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Requested From: United States
Date Requested: 9/22/2019