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IHCPlus™ ATP2A2 / SERCA2 Antibody aa658‑671 Polyclonal IHC,Peptide‑ELISA,WB LS‑B10343

Catalog Number Size Price
LS-B10343-50 50 µg (0.5 mg/ml) $425 
Anti-ATP2A2 / SERCA2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ATP2A2 antibody (0.1 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-ATP2A2 / SERCA2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ATP2A2 antibody (0.1 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ ATP2A2 / SERCA2 Antibody aa658‑671 Polyclonal IHC,Peptide‑ELISA,WB LS‑B10343

Note: This antibody replaces LS-C205061
Antibody:
Goat Polyclonal to Human ATP2A2 / SERCA2
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B10343-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human ATP2A2 / SERCA2
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
SERCA2 antibody LS-B10343 is an unconjugated goat polyclonal antibody to SERCA2 (ATP2A2 ) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ATP2A2 / SERCA2
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence DELNPSAQRDACLN, from the internal region of the protein sequence according to NP_001672.1NP_733765.1.
Blocking Peptide
LS-E46804 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B10343. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa658-671
Specificity
Human ATP2A2 / SERCA2. This antibody is expected to recognize both reported isoforms (NP_001672.1; NP_733765.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 110kD band observed in Human, Mouse and Rat Heart lysates (calculated MW of 110kD according to NP_001672.1). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATP2A2 / SERCA2
P16615 NM_001681 NP_001672.1

Validation

Anti-ATP2A2 / SERCA2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-ATP2A2 / SERCA2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ATP2A2 / SERCA2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 9/16/2019