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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B10343-50 50 µg (0.5 mg/ml) $425 
ATP2A2 / SERCA2 Antibody - Anti-ATP2A2 / SERCA2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ATP2A2 / SERCA2 Antibody - ATP2A2 antibody (0.1 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ATP2A2 / SERCA2 Antibody - Anti-ATP2A2 / SERCA2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ATP2A2 / SERCA2 Antibody - ATP2A2 antibody (0.1 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ Polyclonal Goat anti‑Human ATP2A2 / SERCA2 Antibody (aa658‑671, IHC, WB) LS‑B10343

IHCPlus™ Polyclonal Goat anti‑Human ATP2A2 / SERCA2 Antibody (aa658‑671, IHC, WB) LS‑B10343

Note: This antibody replaces LS-C205061
Antibody:
Goat Polyclonal to Human ATP2A2 / SERCA2
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B10343-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Goat Polyclonal to Human ATP2A2 / SERCA2
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
SERCA2 antibody LS-B10343 is an unconjugated goat polyclonal antibody to SERCA2 (ATP2A2) (aa658-671) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ATP2A2 / SERCA2
Synonyms
ATP2A2 | ATP2B | Calcium pump 2 | DAR | SR Ca(2+)-ATPase 2 | Cardiac Ca2+ ATPase | DD | SERCA2
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence DELNPSAQRDACLN, from the internal region of the protein sequence according to NP_001672.1NP_733765.1.
Blocking Peptide
LS-E46804 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B10343. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa658-671
Specificity
Human ATP2A2 / SERCA2. This antibody is expected to recognize both reported isoforms (NP_001672.1; NP_733765.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 110kD band observed in Human, Mouse and Rat Heart lysates (calculated MW of 110kD according to NP_001672.1). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATP2A2 / SERCA2
P16615 NM_001681 NP_001672.1

Validation

ATP2A2 / SERCA2 Antibody - Anti-ATP2A2 / SERCA2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ATP2A2 / SERCA2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ATP2A2 / SERCA2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ ATP2A2 / SERCA2 Antibody (aa658-671) for IHC, WB/Western LS-B10343 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 7/12/2020