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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B7227-50 50 µg (0.5 mg/ml) $425 
ATP1B1 Antibody - Anti-ATP1B1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ATP1B1 Antibody - ATP1B1 antibody (0.1 ug/ml) staining of Rat Retina lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ATP1B1 Antibody - Anti-ATP1B1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ATP1B1 Antibody - ATP1B1 antibody (0.1 ug/ml) staining of Rat Retina lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ ATP1B1 Antibody aa85‑99 Polyclonal IHC,Peptide‑ELISA,WB LS‑B7227

IHCPlus™ ATP1B1 Antibody aa85‑99 Polyclonal IHC,Peptide‑ELISA,WB LS‑B7227

Note: This antibody replaces LS-C112982
Antibody:
Goat Polyclonal to Human ATP1B1
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Dog, Hamster, Horse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B7227-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human ATP1B1
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Dog, Hamster, Horse
Format:
Unconjugated, Unmodified

Specifications

Description
ATP1B1 antibody LS-B7227 is an unconjugated goat polyclonal antibody to ATP1B1 from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ATP1B1
Host
Goat
Reactivity
Human, Mouse, Rat, Dog, Hamster, Horse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-KTEISFRPNDPKSYE, from the internal region of the protein sequence according to NP_001668.1NP_001001787.1.
Blocking Peptide
LS-E27172 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B7227. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa85-99
Specificity
Human ATP1B1. This antibody is expected to recognize both reported isoforms (NP_001668.1; NP_001001787.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 48kDa band observed in Rat Retina lysates and 50kDa in Mouse and Rat Kidney lysates (calculated MW of 35.1kDa according to Human NP_001668.1, 35.2kDa according to Mouse NP_033851.1 and Rat NP_037245.2).The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATP1B1
P05026 NM_001677 NP_001668.1

Validation

ATP1B1 Antibody - Anti-ATP1B1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ATP1B1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ATP1B1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 11/14/2019