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Catalog Number	Size	Price
LS-B3517-50	50 µl (0.5 mg/ml)	$460

ATF4 Antibody - Anti-ATF4 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

ATF4 Antibody - Anti-ATF4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

ATF4 Antibody - Anti-ATF4 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

ATF4 Antibody - ATF4 antibody Western blot of NIH/3T3 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Polyclonal Rabbit anti‑Mouse ATF4 Antibody (N‑Terminus, IHC, WB) LS‑B3517

Name: Anti-ATF4 Antibody | Rabbit anti-Mouse Polyclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-B3517-50
Price: 460.0000 USD
Availability: InStock
Rating: 4.1 (1 reviews)

(4.1) Rating

Submit Review (2) Publication(s) LSBio Guarantee

Note: This antibody replaces LS-C30114

Antibody:

ATF4 Rabbit anti-Mouse Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Mouse, Human

Format:

Unconjugated, Unmodified

Other formats:

Biotin FITC HRP

$460

LS-B3517-50

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ATF4 Rabbit anti-Mouse Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Mouse, Human

Format:

Unconjugated, Unmodified

Other formats:

Biotin FITC HRP

Specifications

Description

ATF4 antibody LS-B3517 is an unconjugated rabbit polyclonal antibody to ATF4 (N-Terminus) from mouse. It is reactive with human and mouse. Validated for IHC and WB. Cited in 2 publications.

(2) Publication(s)

Target

Mouse ATF4

Synonyms

See All ATF4 Antibodies

Host

Rabbit

Reactivity

Mouse, Human (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated. Also available conjugated with Biotin, FITC, HRP.

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

Synthetic peptide from N-Terminus of mouse ATF4 (Q61328, NP_033846). Percent identity by BLAST analysis: Mouse (100%).

Epitope

N-Terminus

Specificity

Mouse ATF4

Applications

IHC
IHC - Paraffin (5 µg/ml)
Western blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: LS-B3517 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3517 was determined to be 5 ug/ml.

Presentation

PBS, 0.09% sodium azide, 2% sucrose

Storage

Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About ATF4

Uniprot: P18848 NCBI: NM_001675 NP_001666.2

LSBio Ratings

ATF4 Antibody (N-Terminus) for IHC, WB/Western LS-B3517 has an LSBio Rating of

(4.1) Rating

Publications (4.1)

Learn more about The LSBio Ratings Algorithm

Publications (2)

A thrombospondin-dependent pathway for a protective ER stress response. Lynch JM, Maillet M, Vanhoutte D, Schloemer A, Sargent MA, Blair NS, Lynch KA, Okada T, Aronow BJ, Osinska H, Prywes R, Lorenz JN, Mori K, Lawler J, Robbins J, Molkentin JD. Cell. 2012 Jun;149:1257-68.

Full Text Article PubMed: 22682248 PMC: PMC3372931

The Transcription Factor ATF4 Promotes Expression of Cell Stress Genes and Cardiomyocyte Death in a Cellular Model of Atrial Fibrillation. Freundt JK, Frommeyer G, Wötzel F, Huge A, Hoffmeier A, Martens S, Eckardt L, Lange PS. BioMed research international. 2018 May;2018:3694362. (Mouse)

Full Text Article PubMed: 30003094 PMC: PMC5996409

Submit a Publication for LS-B3517