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IHCPlus™ ARSE / Arylsulfatase E Antibody aa504‑553 Polyclonal IHC,WB LS‑B12701

Catalog Number Size Price
LS-B12701-100 100 µl $485 
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ARSE antibody western blot of Human Placenta lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ARSE antibody western blot of Human Placenta lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ ARSE / Arylsulfatase E Antibody aa504‑553 Polyclonal IHC,WB LS‑B12701

Note: This antibody replaces LS-C101923
Antibody:
Rabbit Polyclonal to Human ARSE / Arylsulfatase E
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B12701-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human ARSE / Arylsulfatase E
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Arylsulfatase E antibody LS-B12701 is an unconjugated rabbit polyclonal antibody to human Arylsulfatase E (ARSE ). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ARSE / Arylsulfatase E
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa504-553 of human ARSE (P51690, NP_000038). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey (92%).
Blocking Peptide
LS-E15334 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B12701. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa504-553
Specificity
Human ARSE
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ARSE / Arylsulfatase E
P51690 NM_000047 NP_000038.2

Validation

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Immunohistochemistry image at a dilution of 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, ELISA
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Gibbon
Applications: IHC, IHC - Paraffin, Western blot
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Gibbon
Applications: IHC, IHC - Paraffin, Western blot
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Gibbon
Applications: IHC, IHC - Paraffin, Western blot

Requested From: United States
Date Requested: 9/19/2019