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| Catalog Number | Size | Price |
|---|---|---|
| LS-B10342-50 | 50 µg (0.5 mg/ml) | $485 |
IHC‑plus™ Polyclonal Goat anti‑Human ARHGAP45 Antibody (aa57‑71, IHC) LS‑B10342
IHC‑plus™ Polyclonal Goat anti‑Human ARHGAP45 Antibody (aa57‑71, IHC) LS‑B10342
Note: This antibody replaces LS-C154963
Antibody:
ARHGAP45 Goat anti-Human Polyclonal (aa57-71) Antibody
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human, Monkey, Bovine, Dog, Hamster
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
ARHGAP45 Goat anti-Human Polyclonal (aa57-71) Antibody
Application:
IHC, IHC-P, Peptide-ELISA
Reactivity:
Human, Monkey, Bovine, Dog, Hamster
Format:
Unconjugated, Unmodified
Specifications
Description
ARHGAP45 antibody LS-B10342 is an unconjugated goat polyclonal antibody to ARHGAP45 (aa57-71) from human. It is reactive with human, bovine, dog and other species. Validated for IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues.
Target
Human ARHGAP45
Synonyms
ARHGAP45 | HLA-HA1 | HMHA1 | Ha-1 | KIAA0223 | HA1
Host
Goat
Reactivity
Human, Monkey, Bovine, Dog, Hamster
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence KATGTLKRPTSLSRH-C, from the N-Terminus of protein sequence according to NP_036424.2.
Epitope
aa57-71
Specificity
Human HMHA1 / HA-1. To avoid confusion please note that this antibody is designed to recognise the whole protein as coded by NP_036424.2 and will not recognise the peptide found eluted from HLA-A*0201 molecules by den Haan et al (Science. 1998 Feb 13;279(5353):1054-7, PMID: 9461441)
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Preliminary experiments gave an approx 200kD band in lysates of cell lines Jurkat and Molt4 after 0.05 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 125kD according to NP_036424.2. The 200kD band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ARHGAP45
Validation
Anti-HMHA1 / HA1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HMHA1 / HA1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ ARHGAP45 Antibody (aa57-71) for IHC LS-B10342 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
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Featured Products
Species:
Human
Applications:
IHC, Western blot, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Source:
Wheat
Tag:
GST, N-terminus
Source:
Human
Tag:
Myc-DDK (Flag)
Source:
Human
Tag:
Myc-DDK (Flag)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
