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IHCPlus™ Apolipoprotein A‑V Antibody Monoclonal ELISA,IHC,WB LS‑B2261

Catalog Number Size Price
LS-B2261-100 100 µl $425 
Anti-APOA5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western blot of anti- human APOA5 monoclonal antibody against human serum total protein (lane 2). Lane 1 is a MW standard.
Detection of Apo A5 in HepG2 cell lysate .
Anti-APOA5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western blot of anti- human APOA5 monoclonal antibody against human serum total protein (lane 2). Lane 1 is a MW standard.
Detection of Apo A5 in HepG2 cell lysate .
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IHCPlus™ Apolipoprotein A‑V Antibody Monoclonal ELISA,IHC,WB LS‑B2261

Note: This antibody replaces LS-C2602, LS-C47130
Antibody:
Mouse Monoclonal to Human Apolipoprotein A-V
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2261-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human Apolipoprotein A-V
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Apolipoprotein A-V antibody LS-B2261 is an unconjugated mouse monoclonal antibody to human Apolipoprotein A-V. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Apolipoprotein A-V
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
1G5G9
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Ni-NTA purified full-length recombinant human APOA5 expressed in E. coli strain BL21 (DE3). Percent identity by BLAST analysis: Bovine (83%); Rat (79%); Mouse (78%).
Specificity
Mouse Monoclonal to Apo A5 (1G5G9).
Usage
Immunohistochemistry: LS-B2261 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2261 was determined to be 5 ug/ml.
Presentation
Ascites, 0.1% sodium azide
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Apolipoprotein A-V
Q6Q788 NM_052968 NP_443200.2

Validation

Anti-APOA5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-APOA5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-APOA5 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Species: Human, Mouse, Rat
Applications: Western blot, ELISA
Western Blot; Sample: Fetal Mouse.
Species: Mouse
Applications: Western blot
Western blot of extracts (35ug) from 9 different cell lines by using anti-APOA5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Species: Human, Monkey, Mouse
Applications: Western blot
Reactivity: Mouse
Range: 0.312-20 ng/ml
Reactivity: Human
Range: 31.25-2000 pg/ml

Requested From: United States
Date Requested: 9/23/2019