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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1588-1 | 1 Each (0.4 mg/ml) | $485 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human ALOX15B / 15‑LOX‑2 Antibody (aa161‑179, IHC, WB) LS‑B1588
IHC‑plus™ Polyclonal Rabbit anti‑Human ALOX15B / 15‑LOX‑2 Antibody (aa161‑179, IHC, WB) LS‑B1588
Note: This antibody replaces LS-C11172, LS-C25233
Antibody:
ALOX15B / 15-LOX-2 Rabbit anti-Human Polyclonal (aa161-179) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
ALOX15B / 15-LOX-2 Rabbit anti-Human Polyclonal (aa161-179) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
15-LOX-2 antibody LS-B1588 is an unconjugated rabbit polyclonal antibody to human 15-LOX-2 (ALOX15B) (aa161-179). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ALOX15B / 15-LOX-2
Synonyms
ALOX15B | 15S-lipoxygenase | 15-lipoxygenase 2 | 15-LOX-2 | 15-LOX-B | Arachidonate 15-lipoxygenase B | Arachidonate 15-lipoxygenase 2
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Human 15-lipoxygenase-2 (15-LO-2) amino acids 161-179 (CLDEKTVEDLELNIKYSTA). Percent identity by BLAST analysis: Human (100%); Gorilla (94%).
Epitope
aa161-179
Specificity
Human 15-lipoxygenase-2 (15-LO-2) amino acids 161-179 (CLDEKTVEDLELNIKYSTA. Antibody did not cross-react with 15-LO-1 in Western testing using recombinant protein.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1588 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1588 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.4, 50% glycerol, 0.1% BSA, 0.02 % sodium azide.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ALOX15B / 15-LOX-2
Validation
Anti-ALOX15B / 15 Lipoxygenase 2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ALOX15B / 15 Lipoxygenase 2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ ALOX15B / 15-LOX-2 Antibody (aa161-179) for IHC, WB/Western LS-B1588 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse
Applications:
IHC, Immunofluorescence, Western blot
Reactivity:
Porcine
Range:
0.31-20 ng/ml
Source:
E. coli
Tag:
6His, N-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
