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IHCPlus™ AIM2 Antibody aa321‑335 Polyclonal IHC,Peptide‑ELISA,WB LS‑B5945

Catalog Number Size Price
LS-B5945-50 50 µg (0.5 mg/ml) $425 
Anti-AIM2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Antibody staining of IFNbeta-treated WI-38 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-AIM2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Antibody staining of IFNbeta-treated WI-38 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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IHCPlus™ AIM2 Antibody aa321‑335 Polyclonal IHC,Peptide‑ELISA,WB LS‑B5945

Note: This antibody replaces LS-C112941
Antibody:
Goat Polyclonal to Human AIM2
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B5945-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human AIM2
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
AIM2 antibody LS-B5945 is an unconjugated goat polyclonal antibody to human AIM2. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human AIM2
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-GEKLQLTSGVHSTIK, from the C Terminus of the protein sequence according to NP_004824.1.
Blocking Peptide
LS-E27303 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5945. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa321-335
Specificity
Human AIM2.
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Preliminary experiments gave an approx. 80kDa band in Human Peripheral Blood Mononucleocytes lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 39.0kDa according to NP_004824.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AIM2
O14862 NM_004833 NP_004824.1

Validation

Anti-AIM2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
IHC Validated
Anti-AIM2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-AIM2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

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Requested From: United States
Date Requested: 9/22/2019