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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3427-200 | 200 µl | $460 |
![ACO2 / Aconitase 2 Antibody - Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma); PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2. Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 ?. Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.](https://lsbio-7d62.kxcdn.com/image2/ihc-plus-aco2-aconitase-2-antibody-aa438-467-ls-b3427/225014_1566178.jpg)
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IHC‑plus™ Polyclonal Rabbit anti‑Human ACO2 / Aconitase 2 Antibody (aa438‑467, IHC, WB) LS‑B3427
IHC‑plus™ Polyclonal Rabbit anti‑Human ACO2 / Aconitase 2 Antibody (aa438‑467, IHC, WB) LS‑B3427
Note: This antibody replaces LS-C97947
Antibody:
ACO2 / Aconitase 2 Rabbit anti-Human Polyclonal (aa438-467) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
ACO2 / Aconitase 2 Rabbit anti-Human Polyclonal (aa438-467) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
Aconitase 2 antibody LS-B3427 is an unconjugated rabbit polyclonal antibody to Aconitase 2 (ACO2) (aa438-467) from human. It is reactive with human, mouse and rat. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ACO2 / Aconitase 2
Synonyms
ACO2 | ACONM | Aconitase 2 | Aconitase 2, mitochondrial | Citrate hydro-lyase | ICRD
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Predicted
Pig, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa438-467
Specificity
This ACO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-467 amino acids from the Central region of human ACO2.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1:1000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3427 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3427 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ACO2 / Aconitase 2
Validation
Anti-ACO2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ACO2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ACO2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-ACO2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ ACO2 / Aconitase 2 Antibody (aa438-467) for IHC, WB/Western LS-B3427 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
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Species:
Human, Monkey
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Source:
Human
Tag:
Myc-DDK (Flag)
Source:
Insect
Tag:
His,N-terminus + GST
Species:
Human
Applications:
Flow Cytometry, Functional Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
