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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B6486-50 50 µg (0.5 mg/ml) $425 
ABRI / ITM2B Antibody - Anti-ITM2B antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
ABRI / ITM2B Antibody - ABRI / ITM2B antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).
ABRI / ITM2B Antibody - ABRI / ITM2B antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).
ABRI / ITM2B Antibody - ITM2B antibody (1 ug/ml) staining of Human Hippocampus lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ABRI / ITM2B Antibody - ABRI / ITM2B antibody (0.3µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
ABRI / ITM2B Antibody - ABRI / ITM2B antibody (1µg/ml) staining of Human Hippocampus lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
ABRI / ITM2B Antibody - ABRI / ITM2B antibody flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
ABRI / ITM2B Antibody - Anti-ITM2B antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
ABRI / ITM2B Antibody - ABRI / ITM2B antibody immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).
ABRI / ITM2B Antibody - ABRI / ITM2B antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).
ABRI / ITM2B Antibody - ITM2B antibody (1 ug/ml) staining of Human Hippocampus lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ABRI / ITM2B Antibody - ABRI / ITM2B antibody (0.3µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
ABRI / ITM2B Antibody - ABRI / ITM2B antibody (1µg/ml) staining of Human Hippocampus lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
ABRI / ITM2B Antibody - ABRI / ITM2B antibody flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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IHCPlus™ ABRI / ITM2B Antibody aa224‑238 Polyclonal IF,IHC,Peptide‑ELISA,WB LS‑B6486

IHCPlus™ ABRI / ITM2B Antibody aa224‑238 Polyclonal IF,IHC,Peptide‑ELISA,WB LS‑B6486

Note: This antibody replaces LS-C112793
Antibody:
Goat Polyclonal to Human ABRI / ITM2B
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bovine, Horse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B6486-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human ABRI / ITM2B
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bovine, Horse
Format:
Unconjugated, Unmodified

Specifications

Description
ITM2B antibody LS-B6486 is an unconjugated goat polyclonal antibody to ITM2B (ABRI ) from human, bovine, horse and other species. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ABRI / ITM2B
Host
Goat
Reactivity
Human, Monkey, Bovine, Horse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 - 3.75 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-HDKETYKLQRRETIK, from the internal region of the protein sequence according to NP_068839.1.
Blocking Peptide
LS-E26970 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B6486. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa224-238
Specificity
Human ITM2B.
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx. 33kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 30.3kDa according to NP_068839.1). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ABRI / ITM2B
Q9Y287 NM_021999 NP_068839.1

Validation

ABRI / ITM2B Antibody - Anti-ITM2B antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-ITM2B antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-ITM2B antibody IHC of human small intestine, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

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Requested From: United States
Date Requested: 11/18/2019