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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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IHCPlus™ ABCB4 / MDR3 Antibody aa252‑301 Polyclonal IHC,WB LS‑B5729

Catalog Number Size Price
LS-B5729-100 100 µl $485 
Anti-ABCB4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ABCB4 antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ABCB4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ABCB4 antibody Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ ABCB4 / MDR3 Antibody aa252‑301 Polyclonal IHC,WB LS‑B5729

Note: This antibody replaces LS-C110652
Antibody:
Rabbit Polyclonal to Human ABCB4 / MDR3
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Rabbit
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B5729-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human ABCB4 / MDR3
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Rabbit
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
MDR3 antibody LS-B5729 is an unconjugated rabbit polyclonal antibody to MDR3 (ABCB4 ) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human ABCB4 / MDR3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa252-301 of human ABCB4 (A4D1D4, NP_000434). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Rabbit (100%); Galago, Panda, Opossum (92%); Bovine (85%).
Blocking Peptide
LS-E15357 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5729. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa252-301
Specificity
Human ABCB4
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ABCB4 / MDR3
P21439 NM_018849 NP_061337.1

Validation

Anti-ABCB4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-ABCB4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ABCB4 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Publications (1)

ABCB4 is frequently epigenetically silenced in human cancers and inhibits tumor growth. Kiehl S, Herkt SC, Richter AM, Fuhrmann L, El-Nikhely N, Seeger W, Savai R, Dammann RH. Scientific reports. 4:6899.

Reviews (0)

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Requested From: United States
Date Requested: 8/24/2019
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