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Catalog Number Size Price
LS-B403-50 50 µl (10 mg/ml) $460 
A2M / Alpha-2-Macroglobulin Antibody - Anti-A2M / Alpha-2-Macroglobulin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
A2M / Alpha-2-Macroglobulin Antibody - Alpha-2-Macroglobulin Polyclonal Antibody-Western blot. Goat anti-Alpha-2-Macroglobulin antibody was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti-goat (1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.
A2M / Alpha-2-Macroglobulin Antibody - Anti-A2M / Alpha-2-Macroglobulin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
A2M / Alpha-2-Macroglobulin Antibody - Alpha-2-Macroglobulin Polyclonal Antibody-Western blot. Goat anti-Alpha-2-Macroglobulin antibody was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti-goat (1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.
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IHC‑plus™ Polyclonal Goat anti‑Human A2M / Alpha‑2‑Macroglobulin Antibody (IHC, WB) LS‑B403

IHC‑plus™ Polyclonal Goat anti‑Human A2M / Alpha‑2‑Macroglobulin Antibody (IHC, WB) LS‑B403

Note: This antibody replaces LS-C19056
Antibody:
A2M / Alpha-2-Macroglobulin Goat anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B403-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
A2M / Alpha-2-Macroglobulin Goat anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Alpha-2-Macroglobulin antibody LS-B403 is an unconjugated goat polyclonal antibody to human Alpha-2-Macroglobulin (A2M). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human A2M / Alpha-2-Macroglobulin
Synonyms
A2M | CPAMD5 | A2MD | FWP007 | Alpha-2-M | Alpha-2-macroglobulin | S863-7
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified IgG
Modifications
Unmodified
Specificity
A2-Macroglobulin [Human Plasma].
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:2000 - 1:20000)
  • Immunoprecipitation
  • ELISA (1:80000 - 1:400000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B403 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B403 was determined to be 2.5 ug/ml.
Presentation
0.02 M potassium phosphate buffer, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About A2M / Alpha-2-Macroglobulin
P01023 NM_000014 NP_000005.2

Validation

A2M / Alpha-2-Macroglobulin Antibody - Anti-A2M / Alpha-2-Macroglobulin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-A2M / Alpha-2-Macroglobulin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-A2M / Alpha-2-Macroglobulin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ A2M / Alpha-2-Macroglobulin Antibody for IHC, WB/Western, IP, ELISA LS-B403 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/14/2024