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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C346725-1 1 ml $475 
Antibody

IgG2ab Antibody (FITC) LS‑C346725

IgG2ab Antibody (FITC) LS‑C346725

Antibody:
Goat Polyclonal to Rat IgG2ab
Application:
IHC, IHC-Fr, ICC, IF, ELISA, ID
Reactivity:
Rat
Format:
FITC, Unmodified
Other formats:
Price
Catalog Number
$475 
LS-C346725-1
1 ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Rat IgG2ab
Application:
IHC, IHC-Fr, ICC, IF, ELISA, ID
Reactivity:
Rat
Format:
FITC, Unmodified
Other formats:

Specifications

Description
IgG2ab antibody LS-C346725 is an FITC-conjugated goat polyclonal antibody to rat IgG2ab. Validated for ELISA, ICC, ID, IF and IHC.
Target
Rat IgG2ab
Host
Goat
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
FITC. Also available Unconjugated or conjugated with Biotin, HRP, TRITC.
Purification
Purified IgG
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • ICC
  • Immunofluorescence
  • ELISA
  • Immunodiffusion
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Pools of purified homogenous IgG2a and IgG2b isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Rat IgG2ab, subclass specific.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b as tested against rat sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Further tested on the level of sensitivity of direct and indirect immunofluorescence staining methods applied to lymphoid cells. When used on vital peripheral blood cells this immunoconjugate does not react with Fc receptors present on cell surface membranes. To identify and measure IgG2, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/19/2019