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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C346803-0.2 0.2 mg $590 
Antibody

IgG2 Fab Antibody (clone NI 6014 (HP 6014), TRITC) LS‑C346803

IgG2 Fab Antibody (clone NI 6014 (HP 6014), TRITC) LS‑C346803

Antibody:
Mouse Monoclonal to Human IgG2 Fab
Application:
IHC, IHC-Fr, ICC, IF, ELISA
Reactivity:
Human
Format:
Tetramethyl Rhodamine Iso-Thiocyanate, Unmodified
Price
Catalog Number
$590 
LS-C346803-0.2
0.2 mg
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human IgG2 Fab
Application:
IHC, IHC-Fr, ICC, IF, ELISA
Reactivity:
Human
Format:
Tetramethyl Rhodamine Iso-Thiocyanate, Unmodified

Specifications

Description
IgG2 Fab antibody LS-C346803 is a TRITC-conjugated mouse monoclonal antibody to human IgG2 Fab. Validated for ELISA, ICC, IF and IHC.
Target
Human IgG2 Fab
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
NI 6014 (HP 6014)
Conjugations
Tetramethyl Rhodamine Iso-Thiocyanate
Purification
Purified IgG
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • ICC
  • Immunofluorescence
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Highly purified monoclonal IgG2 isolated from human serum.
Specificity
Human IgG2, subclass specific.
Usage
The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab part of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labeled monoclonal antibody against TRITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50. in ELISA from 1:200 upwards. in Western blotting from 1:400 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
Reconstitute with distilled water.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/18/2019