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Catalog Number Size Price
LS-C346601-1 1 ml $475 
Antibody

IgG2 Antibody (HRP) LS‑C346601

IgG2 Antibody (HRP) LS‑C346601

Antibody:
Goat Polyclonal to Mouse IgG2
Application:
IHC, IHC-P, ICC, ELISA, DB
Reactivity:
Mouse
Format:
Horseradish Peroxidase, Unmodified
Other formats:
Price
Catalog Number
$475 
LS-C346601-1
1 ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Mouse IgG2
Application:
IHC, IHC-P, ICC, ELISA, DB
Reactivity:
Mouse
Format:
Horseradish Peroxidase, Unmodified
Other formats:

Specifications

Description
IgG2 antibody LS-C346601 is an HRP-conjugated goat polyclonal antibody to mouse IgG2. Validated for DB, ELISA, ICC and IHC.
Target
Mouse IgG2
Host
Goat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with Biotin, FITC, TRITC.
Purification
Purified IgG
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • ELISA
  • Dot Blot
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Pools of purified homogenous IgG2a and IgG2b isolated from pooled mouse sera belonging to the allelic types Igh-1a and Igh-1b. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Mouse IgG2, subclass specific.
Usage
The reactivity of the antiserum is directed to the subclass IgG2a and IgG2b. It reacts with determinants specific for IgG2a and IgG2b and iso and allospeciific determinants. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure. in non-isotopic assay methodology (e. g. ELISA) to measure IgG2 in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5000 depending on the method used. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/19/2019