(tested or 100% immunogen sequence identity)
- IHC (15 µg/ml)
- Western blot (0.1 - 0.2 µg/ml)
- ELISA (0.5 - 1 µg/ml)
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IGFBP5 antibody was raised against purified, NS0-derived, recombinant human insulin-like growth factor binding protein 5.
Recognizes human IGFBP5. Recognizes rhIGFBP-5 in direct ELISAs and western blots. In Direct ELISA and Western Blot. Shows approximately 25% cross-reactivity with rmIGFBP-5 and less than 5% cross-reactivity with rhIGFBP-1, rhIGFBP-2, rhIGFBP-3, rhIGFBP-4,
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Western blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human IGFBP-5. The detection limit for rhIGFBP-5 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 15 ug/ml. Detects IGFBP-5 in cells and tissues. Direct ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect human IGFBP-5. The detection limit for rhIGFBP-5 is approximately 0.2 ng/well. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.2. No preservative added. Conjugated to Biotin
100 µl Sterile 40-50% glycerol
Short term: 4°C. Long term: Aliquot and add glycerol (40-50%). Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.