Human, Mouse, Bovine, Pig
(tested or 100% immunogen sequence identity)
- Western blot (1:500 - 1:1000)
IGFBP4 antibody was raised against hPLC purified IGFBP-4 isolated from conditioned medium from the human glioblastoma cell line, T98G.
Recognizes human IGFBP-4 at 24kD. Cross-reactivity: IGFBP-1:1%, IGFBP-2:50%, IGFBP-3: < 0.2%, IGFBP-5: < 0.1%. Species cross-reactivity: Bovine, porcine and mouse.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:500-1:1000 detected human IGFBP-4 in a concentrated serum-free T98G culture supernatant. Immunoprecipitation: 4 ul immunoprecipitated >90% of 10 ng IGFBP-4 from 500 ul concentrated serum-free T98G culture supernatant.
200 µl deionized water
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.