Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IGFBP4, BP-4, HT29-IGFBP, IGF-binding protein 4, IBP-4, IGFBP-4, IBP4
Human, Mouse, Bovine, Pig
(tested or 100% immunogen sequence identity)
Western blot (1:500 - 1:1000)
IGFBP4 antibody was raised against hPLC purified IGFBP-4 isolated from conditioned medium from the human glioblastoma cell line, T98G.
Recognizes human IGFBP-4 at 24kD. Cross-reactivity: IGFBP-1:1%, IGFBP-2:50%, IGFBP-3: < 0.2%, IGFBP-5: < 0.1%. Species cross-reactivity: Bovine, porcine and mouse.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:500-1:1000 detected human IGFBP-4 in a concentrated serum-free T98G culture supernatant. Immunoprecipitation: 4 ul immunoprecipitated >90% of 10 ng IGFBP-4 from 500 ul concentrated serum-free T98G culture supernatant.
200 µl deionized water
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.