Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C407922-10 10 µg $318 
LS-C407922-100 100 µg $470 
IGFBP3 Antibody - IGFBP3 antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.
IGFBP3 Antibody - Western blot analysis of IGFBP-3 using anti-IGFBP-3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat PC-12 whole cell lysates. Lane 5: human U-87MG whole cell lysates. Lane 6: mouse kidney tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP-3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP-3 at approximately 40KD. The expected band size for IGFBP-3 is at 32KD.
IGFBP3 Antibody - IGFBP3 antibody Western blot. All lanes: Anti IGFBP3 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: SGC Whole Cell Lysate at 40 ug. Lane 4: 22RV1 Whole Cell Lysate at 40 ug. Predicted band size: 31 kD. Observed band size: 31 kD.
IGFBP3 Antibody - IGFBP3 antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.
IGFBP3 Antibody - Western blot analysis of IGFBP-3 using anti-IGFBP-3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: rat PC-12 whole cell lysates. Lane 5: human U-87MG whole cell lysates. Lane 6: mouse kidney tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP-3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP-3 at approximately 40KD. The expected band size for IGFBP-3 is at 32KD.
IGFBP3 Antibody - IGFBP3 antibody Western blot. All lanes: Anti IGFBP3 at 0.5 ug/ml. Lane 1: Rat Kidney Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: SGC Whole Cell Lysate at 40 ug. Lane 4: 22RV1 Whole Cell Lysate at 40 ug. Predicted band size: 31 kD. Observed band size: 31 kD.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human IGFBP3 Antibody (aa214‑252, IHC, WB) LS‑C407922

Polyclonal Rabbit anti‑Human IGFBP3 Antibody (aa214‑252, IHC, WB) LS‑C407922

Antibody:
IGFBP3 Rabbit anti-Human Polyclonal (aa214-252) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407922-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IGFBP3 Rabbit anti-Human Polyclonal (aa214-252) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
IGFBP3 antibody LS-C407922 is an unconjugated rabbit polyclonal antibody to IGFBP3 (aa214-252) from human. It is reactive with human and rat. Validated for ELISA, IHC and WB. Cited in 1 publication.
Target
Human IGFBP3
Synonyms
IGFBP3 | Binding protein 29 | Binding protein 53 | BP-53 | IGF-binding protein 3 | IGFBP-3 | IBP-3 | IBP3
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human IGFBP-3 (214-252 aa RREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCR), identical to the related mouse and rat sequences.
Epitope
aa214-252
Specificity
Expressed by most tissues. Present in plasma.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IGFBP3
P17936 NM_000598 NP_000589.2

LSBio Ratings

IGFBP3 Antibody (aa214-252) for IHC, WB/Western, ELISA LS-C407922 has an LSBio Rating of
Publications (4)

Publications (1)

EZH1 and EZH2 promote skeletal growth by repressing inhibitors of chondrocyte proliferation and hypertrophy. Lui JC, Garrison P, Nguyen Q, Ad M, Keembiyehetty C, Chen W, Jee YH, Landman E, Nilsson O, Barnes KM, Baron J. Nature communications. 2016 7:13685. (WB; Human)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024