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Catalog Number Size Price
LS-C662038-10 10 µg $318 
LS-C662038-100 100 µg $470 
IGFBP1 Antibody - Immunohistochemistry - Anti-IGFBP1 Picoband Antibody
IGFBP1 Antibody - Immunohistochemistry - Anti-IGFBP1 Picoband Antibody
IGFBP1 Antibody - Immunohistochemistry - Anti-IGFBP1 Picoband Antibody
IGFBP1 Antibody - Immunohistochemistry - Anti-IGFBP1 Picoband Antibody
IGFBP1 Antibody - Western blot analysis of IGFBP1 using anti-IGFBP1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP1 at approximately 30KD. The expected band size for IGFBP1 is at 28KD.
IGFBP1 Antibody - Western blot - Anti-IGFBP1 Picoband Antibody
IGFBP1 Antibody - Immunohistochemistry - Anti-IGFBP1 Picoband Antibody
IGFBP1 Antibody - Immunohistochemistry - Anti-IGFBP1 Picoband Antibody
IGFBP1 Antibody - Immunohistochemistry - Anti-IGFBP1 Picoband Antibody
IGFBP1 Antibody - Immunohistochemistry - Anti-IGFBP1 Picoband Antibody
IGFBP1 Antibody - Western blot analysis of IGFBP1 using anti-IGFBP1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP1 at approximately 30KD. The expected band size for IGFBP1 is at 28KD.
IGFBP1 Antibody - Western blot - Anti-IGFBP1 Picoband Antibody
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Polyclonal Rabbit anti‑Mouse IGFBP1 Antibody (IHC, WB) LS‑C662038

Polyclonal Rabbit anti‑Mouse IGFBP1 Antibody (IHC, WB) LS‑C662038

Antibody:
IGFBP1 Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662038-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IGFBP1 Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
IGFBP1 antibody LS-C662038 is an unconjugated rabbit polyclonal antibody to IGFBP1 from mouse. It is reactive with mouse and rat. Validated for ELISA, IHC and WB.
Target
Mouse IGFBP1
Synonyms
IGFBP1 | AFBP | Amniotic fluid binding protein | Binding protein-26 | Binding protein-25 | Binding protein-28 | IGF-binding protein 1 | IGF-BP25 | IBP1 | HIGFBP-1 | PP12 | Placental protein 12 | IBP-1 | IGFBP-1
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived mouse IGFBP1 recombinant protein (Position: A26-N272).
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (1 - 5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IGFBP1
P08833 NM_000596 NP_000587.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024