(tested or 100% immunogen sequence identity)
Ion exchange chromatography
- Western blot (0.1 - 0.2 µg/ml)
- Functional Assay
IGF2 antibody was raised against highly purified recombinant human IGF-II.
Recognizes Insulin-Like Growth Factor II (IGF-II).
Suitable for use in ELISA, Neutralization and Western Blot. Direct ELISA: < 0.5 ug detects 0.2-0.4 ng/well of recombinant hIGF-II using 100 ul/well antibody solution. Neutralization: 3.1-4.6 mg/ml yields one-half maximal inhibition [ND50] of the biological activity of hIGF-II (10 ng/ml). Western Blot: 0.1-0.2 ug/ml detects recombinant hIGF-II at 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.2, 0.1% Sodium Azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
For research use only.