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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C347037-1 1 ml $475 
Antibody

IgA + IgG1 + IgG2a + IgG2b + IgG2c Antibody (HRP) LS‑C347037

IgA + IgG1 + IgG2a + IgG2b + IgG2c Antibody (HRP) LS‑C347037

Antibody:
Rabbit Polyclonal to Rat IgA + IgG1 + IgG2a + IgG2b + IgG2c
Application:
IHC, IHC-P, ICC, ELISA, DB
Reactivity:
Rat
Format:
Horseradish Peroxidase, Unmodified
Other formats:
Price
Catalog Number
$475 
LS-C347037-1
1 ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Rat IgA + IgG1 + IgG2a + IgG2b + IgG2c
Application:
IHC, IHC-P, ICC, ELISA, DB
Reactivity:
Rat
Format:
Horseradish Peroxidase, Unmodified
Other formats:

Specifications

Description
IgA + IgG1 + IgG2a + IgG2b + IgG2c antibody LS-C347037 is an HRP-conjugated rabbit polyclonal antibody to rat IgA + IgG1 + IgG2a + IgG2b + IgG2c. Validated for DB, ELISA, ICC and IHC.
Target
Rat IgA + IgG1 + IgG2a + IgG2b + IgG2c
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with Biotin, FITC.
Purification
Purified IgG
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • ELISA
  • Dot Blot
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified polyclonal rat IgG, and homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Rat IgG, IgA and IgM, heavy and light chains.
Usage
The reactivity of the antiserum is directed to the major isotypes of the rat immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rat plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. In enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. The presence of activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents or GARa/Ig(Fc)/PO should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:250 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:10000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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