Rat IgA + IgG1 + IgG2a + IgG2b + IgG2c

Rabbit

Rat (tested or 100% immunogen sequence identity)

Polyclonal

FITC. Also available Unconjugated or conjugated with Biotin, HRP.

Purified IgG

Unmodified

Purified polyclonal rat IgG, including all subclasses, and pools of homogenous immunoglobulins. Freund's complete adjuvant is used in the first step of the immunization procedure.

Rat immunoglobulins, heavy and light chains.

• IHC
• IHC - Frozen
• ICC
• Immunofluorescence
• ELISA
• Applications tested for the base form of this product only

The reactivity of the antiserum is directed to all known isotypes of the rat immunoglobulin system (classes, subclasses and light chain types) including antibodies to common determinants, to class and subclass specific determinants, and to the surface determinants of the common Fab portion as tested by immunoelectrophoresis and double radial immunodiffusion. It does not react with any non-Ig protein in rat serum. To identify rat immunoglobulins, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from PBS, pH 7.2, No preservatives added

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.