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Catalog Number Size Price
LS-C347379-1 1 ml $453 
Antibody

Polyclonal Goat anti‑Rat IgA + IgG1 + IgG2a + IgG2b + IgG2c Antibody (Biotin, IHC) LS‑C347379

Polyclonal Goat anti‑Rat IgA + IgG1 + IgG2a + IgG2b + IgG2c Antibody (Biotin, IHC) LS‑C347379

Antibody:
IgA + IgG1 + IgG2a + IgG2b + IgG2c Goat anti-Rat Polyclonal (Biotin) Antibody
Application:
IHC, IHC-P, ICC, ELISA, DB
Reactivity:
Rat
Format:
Biotin, Unmodified
Other formats:
Price
Catalog Number
$453
LS-C347379-1
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
IgA + IgG1 + IgG2a + IgG2b + IgG2c Goat anti-Rat Polyclonal (Biotin) Antibody
Application:
IHC, IHC-P, ICC, ELISA, DB
Reactivity:
Rat
Format:
Biotin, Unmodified
Other formats:

Specifications

Description
IgA + IgG1 + IgG2a + IgG2b + IgG2c antibody LS-C347379 is a biotin-conjugated goat polyclonal antibody to rat IgA + IgG1 + IgG2a + IgG2b + IgG2c. Validated for DB, ELISA, ICC and IHC.
Target
Rat IgA + IgG1 + IgG2a + IgG2b + IgG2c
Host
Goat
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with FITC, HRP, TRITC.
Purification
Purified IgG
Modifications
Unmodified
Immunogen
Highly purified IgG and pools of homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Rat IgG, IgA and IgM, heavy and light chains.
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • ELISA
  • Dot Blot
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The reactivity of the antiserum is directed to the major isotypes of the rat immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rat plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. In immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates. to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of rat origin in the middle layer of the indirect test procedure. in non-isotopic assay methodology (e. g. ELISA) to measure immunoglobulins in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:5000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/23/2022