Rat IgA + IgG1 + IgG2a + IgG2b + IgG2c
(tested or 100% immunogen sequence identity)
Also available Unconjugated
or conjugated with HRP
- IHC - Paraffin
- Dot Blot
- Applications tested for the base form of this product only
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Purified polyclonal IgG, including all subclasses, and pools of homogenous IgA, IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Rat IgG, IgA and IgM, heavy and light chains.
The reactivity of the antiserum is directed the major immunoglobulin isotypes of the rat Ig system (classes, subclasses and L-chain types), including antibodies to common determinants, to class and subclass-specific determinants, and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rat plasma and serum. In immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates. to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of rat origin in the middle layer of the indirect test procedure. in non-isotopic assay methodology (e. g. ELISA) to measure immunoglobulins in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500. in ELISA and comparable non-precipitating antibody-binding assays between 1:2000 and 1:10000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized from PBS, pH 7.2, No preservatives added
1 ml Distilled water
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
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