Rat Ig Kappa + Lambda Light Chain
(tested or 100% immunogen sequence identity)
Purified light chains isolated from polyclonal rat IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.
Rat light chains, free and bound.
As tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal rat serum and purified IgG fractions and light chain preparations, the antiserum reacts with determinants exposed on the surface (SD) and the Fab portion of all intact immunoglobulins, with Fab or F(ab')2 subunits, and with free light chains of both types. also with determinants exposed only on free light chains but hidden in the intact immunoglobulin molecule (HD or hidden determinants) or Bence Jones proteins. This antiserum is not kappa or lambda chain specific. It does not react with any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As precipitating antiserum in a variety of immunodiffusion techniques including immunoelectrophoresis and double and single radial immunodiffusion Mancini, Ouchterlony). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.
Delipidated, heat-inactivated, lyophilized antiserum. No preservative.
1 ml Distilled water
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.