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Catalog Number	Size	Price
LS-C153837-100	100 µg (1 mg/ml)	$574

IDO1 / IDO Antibody - Immunofluorescence Microscopy of Mouse Anti-IDO1 Antibody. Cells: HEK293 cells. Fixation: 0.5% PFA. Expressing: mouse IDO-1 (left) and mouse IDO-2 (right). Primary antibody: IDO1 (2E2) monoclonal antibody. Antigen retrieval: not required. Secondary antibody: mouse secondary antibody at 1:10000 for 45 min at RT. Localization: IDO-1 is located in the cytosol. Staining: IDO1 as red fluorescent signal with bis-benzimide nuclear counterstain (blue).

IDO1 / IDO Antibody - Western Blot of mouse anti-IDO1 antibody. Lane 1: HEK293 control vector. Lane 2: HEK293 expressing mouse IDO1. Lane 3: HEK293 expressing mouse IDO2. Load: 35 µg per lane. Primary antibody: IDO 1 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 45.6 kDa, ~44 kDa for IDO1. Other band(s): non-specifics.

IDO1 / IDO Antibody - Western Blot of Mouse Anti-IDO1 Antibody. Extracts from 293HEK Cells expressing: Lane 1: Control Vector. Lane 2: His-tagged mouse IDO1. Lane 3: mouse IDO1. Lane 4: His-tagged mouse IDO2. Lane 5: mouse IDO2. Lane 6: Epididymis from IDO null. Lane 7: wild type mice. Primary antibody: IDO-1(2E2) monoclonal antibody. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBST overnight at 4°C. Predicted/Observed size: 41-42 kDa/41-42 kDa for IDO-1. Other band(s): none.

IDO1 / IDO Antibody - Flow Cytometry of Mouse Anti-IDO1 antibody. Cells: HEK293 cells. Expresing: mouse IDO-1(blue) and mouse IDO-2 (red). Primary antibody: IDO1 (2E2) monoclonal antibody. Secondary antibody: Biotin mouse secondary antibody at 1:10,000 for 45 min at RT and streptavidin PE at 1:5,000 for 30 min at RT.

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Monoclonal Mouse anti‑Mouse IDO1 / IDO Antibody (clone 2E2.6, IHC, IF, WB) LS‑C153837

Name: Anti-IDO1 / IDO Antibody | Mouse anti-Mouse Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C153837-100
Price: 574.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

(4.0) Rating

Submit Review (1) Publication(s) LSBio Guarantee

Antibody:

IDO1 / IDO Mouse anti-Mouse Monoclonal (2E2.6) Antibody

Application:

IHC, ICC, IF, WB, Flo, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

$574

LS-C153837-100

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

IDO1 / IDO Mouse anti-Mouse Monoclonal (2E2.6) Antibody

Application:

IHC, ICC, IF, WB, Flo, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

IDO antibody LS-C153837 is an unconjugated mouse monoclonal antibody to mouse IDO (IDO1). Validated for ELISA, Flow, ICC, IF, IHC and WB. Cited in 1 publication.

(1) Publication(s)

Target

Mouse IDO1 / IDO

Synonyms

See All IDO1 / IDO Antibodies

Host

Mouse

Reactivity

Mouse (tested or 100% immunogen sequence identity)

Non-Reactivity

Human

Clonality

IgG1 Monoclonal

Clone

2E2.6

Conjugations

Unconjugated

Purification

Protein A affinity chromatography

Modifications

Unmodified

Immunogen

IDO1 antibody was produced in mouse by repeated immunizations with mouse recombinant IDO1 protein followed by hybridoma development.

Specificity

Mouse IDO1 does not react with human tissues. Cross-reactivity with IDO1 from other sources has not been determined.

Applications

IHC
ICC
Immunofluorescence (1:50 - 1:100)
Western blot (1:500 - 1:1500)
Flow Cytometry
ELISA (1:5000 - 1:50000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Anti-IDO1 antibody has been tested for use in ELISA, Western Blot, IF, IHC, and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About IDO1 / IDO

Uniprot: P14902 NCBI: NM_002164 AAA36081.1

LSBio Ratings

IDO1 / IDO Antibody (clone 2E2.6) for IHC, ICC, IF/Immunofluorescence, WB/Western, Flow, ELISA LS-C153837 has an LSBio Rating of

(4.0) Rating

Publications (4)

Learn more about The LSBio Ratings Algorithm

Publications (1)

Regulation of indoleamine 2, 3-dioxygenase in hippocampal microglia by NLRP3 inflammasome in lipopolysaccharide-induced depressive-like behaviors. Shanshan Zhang, Ying Zong, Zhonggan Ren, Juntao Hu, Xinyuan Wu, Honglei Xiao, Song Qin, Guomin Zhou, Yuanyuan Ma, Yaodong Zhang, Jin Yu, Kaidi Wang, Guocai Lu, Qiong Liu. The European journal of neuroscience. 2020 December;52:4586-4601.

Full Text Article PubMed: 33098156

Submit a Publication for LS-C153837