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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IDE Antibody LS‑C109461
IDE antibody LS-C109461 is an unconjugated rabbit polyclonal antibody to human IDE. Validated for WB.
Catalog
Size
Price
LS-C109461-100
100 µl
Unavailable

Popular IDE Products

Antibody (1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Anti-IDE antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3954 concentration 2.5 ug/ml.
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Anti-IDE antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4179 concentration 3.75 ug/ml.
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Species: Human
Applications: IHC, Western blot, ELISA
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Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

IDE antibody LS-C109461 is an unconjugated rabbit polyclonal antibody to human IDE. Validated for WB.
About IDE
IDE is a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. P14735 NM_004969 NP_004960.2

IDE Antibody, Abeta-degrading protease Antibody, Insulinase Antibody, INSULYSIN Antibody, Insulin protease Antibody, Insulin-degrading enzyme Antibody


Specifications

Target
Human IDE
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:3000)
Failed Applications
  • IHC - Paraffin
Immunogen
IDE antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 5 and 235 of Human insulin-degrading enzyme.
Specificity
Human insulin degrading enzyme. Predicted cross-reactivity based on amino acid sequence homology: mouse (98%), rat (98%), bovine (98%), zebrafish (90%).
Presentation
0.1 M Tris-glycine, pH 7, 10% Glycerol, 0.01% Thimerosal
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. IDE antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. IDE antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. IDE antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. IDE antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. IDE antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. IDE antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. IDE antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. IDE antibody diluted at 1:1000.

Requested From: 
Date Requested: 7/18/2018

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