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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ICSBP / IRF8 Antibody IHC-plus™ LS-B578

Note: This antibody replaces LS-C458
IRF8 antibody LS-B578 is an unconjugated rabbit polyclonal antibody to IRF8 (ICSBP) from human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B578-0.05
0.05 mg
$425
Description
IRF8 antibody LS-B578 is an unconjugated rabbit polyclonal antibody to IRF8 (ICSBP) from human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ICSBP / IRF8
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ICSBP / IRF8 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRF8. The immunogen is located within the last 50 amino acids of IRF8.
Blocking Peptide
LS-E30095 - Liquid - 0.05 mg - $145.00
Immunizing peptide used to generate LS-B578. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Usage
IRF8 antibody can be used for detection of IRF8 by Western blot at 1 ug/mL. For immunofluorescence start at 10 ug/mL. Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About ICSBP / IRF8
Q02556 NM_002163 NP_002154.1

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Images

Immunohistochemistry

Anti-IRF8 antibody IHC of human small intestine, Peyer's patch. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IRF8 antibody IHC of human small intestine, Peyer's patch. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 ug/ml.
Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 ug/ml.

Western blot

Western blot of IRF8 in human thymus tissue lysate with IRF8 antibody at 1ug/ml in (A) the presence and (B) absence of blocking peptide.
Western blot of IRF8 in human thymus tissue lysate with IRF8 antibody at 1ug/ml in (A) the presence and (B) absence of blocking peptide.

Immunohistochemistry

Anti-IRF8 antibody IHC of human small intestine, Peyer's patch. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IRF8 antibody IHC of human small intestine, Peyer's patch. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 ug/ml.
Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 ug/ml.

Western blot

Western blot of IRF8 in human thymus tissue lysate with IRF8 antibody at 1ug/ml in (A) the presence and (B) absence of blocking peptide.
Western blot of IRF8 in human thymus tissue lysate with IRF8 antibody at 1ug/ml in (A) the presence and (B) absence of blocking peptide.

Immunohistochemistry

Anti-IRF8 antibody IHC of human small intestine, Peyer's patch. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IRF8 antibody IHC of human small intestine, Peyer's patch. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 ug/ml.
Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 ug/ml.

Western blot

Western blot of IRF8 in human thymus tissue lysate with IRF8 antibody at 1ug/ml in (A) the presence and (B) absence of blocking peptide.
Western blot of IRF8 in human thymus tissue lysate with IRF8 antibody at 1ug/ml in (A) the presence and (B) absence of blocking peptide.

Immunohistochemistry

Anti-IRF8 antibody IHC of human small intestine, Peyer's patch. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IRF8 antibody IHC of human small intestine, Peyer's patch. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 ug/ml.
Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 ug/ml.

Western blot

Western blot of IRF8 in human thymus tissue lysate with IRF8 antibody at 1ug/ml in (A) the presence and (B) absence of blocking peptide.
Western blot of IRF8 in human thymus tissue lysate with IRF8 antibody at 1ug/ml in (A) the presence and (B) absence of blocking peptide.

Requested From: United States
Date Requested: 2/19/2019
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