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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ICSBP / IRF8 Antibody (C‑Terminus, FITC) LS‑C426241
IRF8 antibody LS-C426241 is an FITC-conjugated rabbit polyclonal antibody to IRF8 (ICSBP) from human, chimpanzee and pig. Validated for WB.
Catalog
Size
Price
LS-C426241-100
100 µl (1 mg/ml)
$385

Popular ICSBP / IRF8 Products

Anti-IRF8 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2127 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-IRF8 antibody IHC of human small intestine, Peyer's patch. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B578 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
IRF8 antibody LS-C29725 was used in IHC to stain formalin-fixed, paraffin-embedded human liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Detection of Mouse IRF8 by Western Blot. Samples: Whole cell lysate from RAW 264.7 (66 and 33 ug ). Antibodies: Affinity purified rabbit anti-IRF8 antibody used for WB at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.
Species: Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit
Applications: Western blot, Immunoprecipitation
Western blot of anti-IRF8 Antibody in HL60 cell line lysates (35 ug/lane). IRF8 (arrow) was detected using the purified antibody.
Species: Human
Applications: Western blot, Flow Cytometry

Product Description

IRF8 antibody LS-C426241 is an FITC-conjugated rabbit polyclonal antibody to IRF8 (ICSBP) from human, chimpanzee and pig. Validated for WB.
About ICSBP / IRF8
Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. Q02556 NM_002163 NP_002154.1

IRF8 Antibody, H-ICSBP Antibody, Interferon regulatory factor 8 Antibody, ICSBP Antibody, ICSBP1 Antibody, IRF-8 Antibody


Specifications

Target
Human ICSBP / IRF8
Host
Rabbit
Reactivity
Human, Chimpanzee, Pig (tested or 100% immunogen sequence identity)
Predicted
Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Fluorescein. Also available Unconjugated or conjugated with Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
ICSBP / IRF8 antibody was raised against synthetic peptide from C-Terminus of human IRF8 (Q02556, NP_002154). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Opossum (100%); Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Guinea pig, Platypus, Xenopus (92%); Galago (85%); Pig (84%).
Epitope
C-Terminus
Specificity
Human IRF8
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.

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Images

Western blot

IRF8 antibody LS-C145527 Western blot of 1 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IRF8 antibody LS-C145527 Western blot of 1 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

IRF8 antibody LS-C145527 Western blot of 1 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IRF8 antibody LS-C145527 Western blot of 1 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

IRF8 antibody LS-C145527 Western blot of 1 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IRF8 antibody LS-C145527 Western blot of 1 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

IRF8 antibody LS-C145527 Western blot of 1 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IRF8 antibody LS-C145527 Western blot of 1 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/24/2018

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