LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
ICSBP / IRF8 Antibody (aa384‑409) LS‑C97321
IRF8 antibody LS-C97321 is an unconjugated rabbit polyclonal antibody to human IRF8 (ICSBP). Validated for Flow and WB.
Catalog
Size
Price
LS-C97321-400
400 µl
$305

Popular ICSBP / IRF8 Products

Anti-IRF8 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2127 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-IRF8 antibody IHC of human small intestine, Peyer's patch. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B578 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
IRF8 antibody LS-C29725 was used in IHC to stain formalin-fixed, paraffin-embedded human liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Detection of Mouse IRF8 by Western Blot. Samples: Whole cell lysate from RAW 264.7 (66 and 33 ug ). Antibodies: Affinity purified rabbit anti-IRF8 antibody used for WB at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.
Species: Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit
Applications: Western blot, Immunoprecipitation

Product Description

IRF8 antibody LS-C97321 is an unconjugated rabbit polyclonal antibody to human IRF8 (ICSBP). Validated for Flow and WB.
About ICSBP / IRF8
Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. Q02556 NM_002163 NP_002154.1

IRF8 Antibody, H-ICSBP Antibody, Interferon regulatory factor 8 Antibody, ICSBP Antibody, ICSBP1 Antibody, IRF-8 Antibody


Specifications

Target
Human ICSBP / IRF8
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Blocking Peptide
LS-E7131 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C97321. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa384-409
Specificity
This IRF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 384-409 amino acids from the C-terminal region of human IRF8.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.

Publications (0)


Customer Reviews (0)


Images

Western blot

Western blot of anti-IRF8 Antibody in HL60 cell line lysates (35 ug/lane). IRF8 (arrow) was detected using the purified antibody.
Western blot of anti-IRF8 Antibody in HL60 cell line lysates (35 ug/lane). IRF8 (arrow) was detected using the purified antibody.

Flow Cytometry

IRF8 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IRF8 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

Western blot of anti-IRF8 Antibody in HL60 cell line lysates (35 ug/lane). IRF8 (arrow) was detected using the purified antibody.
Western blot of anti-IRF8 Antibody in HL60 cell line lysates (35 ug/lane). IRF8 (arrow) was detected using the purified antibody.

Flow Cytometry

IRF8 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IRF8 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

Western blot of anti-IRF8 Antibody in HL60 cell line lysates (35 ug/lane). IRF8 (arrow) was detected using the purified antibody.
Western blot of anti-IRF8 Antibody in HL60 cell line lysates (35 ug/lane). IRF8 (arrow) was detected using the purified antibody.

Flow Cytometry

IRF8 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IRF8 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

Western blot of anti-IRF8 Antibody in HL60 cell line lysates (35 ug/lane). IRF8 (arrow) was detected using the purified antibody.
Western blot of anti-IRF8 Antibody in HL60 cell line lysates (35 ug/lane). IRF8 (arrow) was detected using the purified antibody.

Flow Cytometry

IRF8 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IRF8 Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 9/25/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy