(tested or 100% immunogen sequence identity)
Pooled whole human milk
In immunoelectrophoresis against human milk precipitation of not less than 7 different proteins may be obtained. However the number of visible precipitin lines varies widely among individual specimens of human milk. The total concentration of protein, their proportion of the different protein components change markedly during the course of lactation. A protein detected in a human secretion by this antiserum may be of secretory origin, or it may be a plasma protein presumably entering in the secretion from the surrounding capillary vessels and through interstitial tissues. Virtually every plasma protein may also be present at a detectable level in milk. Not all precipitable proteins have been identified. Known proteins include secretory IgA and other immunoglobulins, free secretory component, lactoferrin, components of the complement system and albumin. Inter-species cross reactivity is a normal feature of antibodies to animal proteins since homologousproteins of different species frequently share antigenic determinants. Not species-specific.
Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). In immunoelectrophoresis use 2 µl serum or equivalent against 120 µl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
No preservative or foreign proteins added
For research use only.
This antibody carries the LSBio 100% Guarantee.