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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HTR1E / 5‑HT1E Receptor Antibody (Cytoplasmic Domain) IHC‑plus™ LS‑A6374 Best Seller
5-HT1E Receptor antibody LS-A6374 is an unconjugated rabbit polyclonal antibody to 5-HT1E Receptor (HTR1E) from human, bat, guinea pig and other species. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A6374-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment
Description
5-HT1E Receptor antibody LS-A6374 is an unconjugated rabbit polyclonal antibody to 5-HT1E Receptor (HTR1E) from human, bat, guinea pig and other species. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human HTR1E / 5-HT1E Receptor
Host
Rabbit
Reactivity
Human, Monkey, Bat, Guinea pig, Horse (tested or 100% immunogen sequence identity)
Predicted
Bovine (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (16 - 24 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human 5HT1E Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Bat, Elephant, Horse, Guinea pig (100%); Orangutan, Gibbon, Bovine (95%); Panda (89%); Opossum (84%).
Blocking Peptide
LS-E29128 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A6374. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Cytoplasmic Domain
Specificity
Human 5HT1E Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A6374 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A6374 was determined to be 16-24 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About HTR1E / 5-HT1E Receptor
P28566 NM_000865 NP_000856.1

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Immunohistochemistry

Anti-5HT1E Receptor antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-5HT1E Receptor antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-5HT1E Receptor antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-5HT1E Receptor antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-5HT1E Receptor antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-5HT1E Receptor antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-5HT1E Receptor antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-5HT1E Receptor antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 12/9/2018
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