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Catalog Number Size Price
LS-C818553-100 100 µl $412 
HSPD1 / HSP60 Antibody - Immunochemical staining of mouse HSP60 in mouse liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
HSPD1 / HSP60 Antibody - Immunochemical staining of mouse HSP60 in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
HSPD1 / HSP60 Antibody - Immunofluorescence staining of mouse HSPD1 in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Mouse HSPD1 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Mitochondrion.
HSPD1 / HSP60 Antibody - Anti-HSP60 rabbit polyclonal antibody at 1:10000 dilution. Lane A: NIH-3T3 Whole Cell Lysate. Lane B: Raw264.7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 60 kDa.
HSPD1 / HSP60 Antibody - mHSP60 was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate. 4 uL anti-mHSP60 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-mHSP60 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 60 kDa.
HSPD1 / HSP60 Antibody - Immunochemical staining of mouse HSP60 in mouse liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
HSPD1 / HSP60 Antibody - Immunochemical staining of mouse HSP60 in mouse kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
HSPD1 / HSP60 Antibody - Immunofluorescence staining of mouse HSPD1 in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Mouse HSPD1 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Mitochondrion.
HSPD1 / HSP60 Antibody - Anti-HSP60 rabbit polyclonal antibody at 1:10000 dilution. Lane A: NIH-3T3 Whole Cell Lysate. Lane B: Raw264.7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 60 kDa.
HSPD1 / HSP60 Antibody - mHSP60 was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate. 4 uL anti-mHSP60 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-mHSP60 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 60 kDa. Observed band size: 60 kDa.
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Polyclonal Rabbit anti‑Mouse HSPD1 / HSP60 Antibody (Preservative Free, IHC, IF, WB) LS‑C818553

Polyclonal Rabbit anti‑Mouse HSPD1 / HSP60 Antibody (Preservative Free, IHC, IF, WB) LS‑C818553

Antibody:
HSPD1 / HSP60 Rabbit anti-Mouse Polyclonal (Preservative Free) Antibody
Application:
IHC-P, ICC, IF, WB, IP, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$412
LS-C818553-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HSPD1 / HSP60 Rabbit anti-Mouse Polyclonal (Preservative Free) Antibody
Application:
IHC-P, ICC, IF, WB, IP, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Preservative Free

Specifications

Description
HSP60 antibody LS-C818553 is an unconjugated rabbit polyclonal antibody to mouse HSP60 (HSPD1). Validated for ELISA, ICC, IF, IHC, IP and WB.
Target
Mouse HSPD1 / HSP60
Synonyms
HSPD1 | 60 kDa chaperonin | CPN60 | Heat shock protein 65 | Heat shock protein 60 | HSP-60 | HSP60 | HuCHA60 | HLD4 | HSP65 | p60 lymphocyte protein | SPG13 | Chaperonin 60 | GROEL
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Preservative Free
Immunogen
Recombinant Mouse HSP60/HSPD1 Protein
Specificity
Mouse HSP60
Applications
  • IHC - Paraffin (1:500 - 1:2000)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:10000 - 1:50000)
  • Immunoprecipitation
  • ELISA (1:5000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPD1 / HSP60
P10809 NM_002156 NP_002147.2

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Requested From: United States
Date Requested: 4/19/2024