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Catalog Number Size Price
LS-C331177-20 20 µl $263 
LS-C331177-50 50 µl $301 
LS-C331177-100 100 µl $359 
LS-C331177-200 200 µl $473 
HSPD1 / HSP60 Antibody - Immunohistochemistry of paraffin-embedded rat kidney using HSPD1 antibodyat dilution of 1:100 (40x lens).
HSPD1 / HSP60 Antibody - Immunofluorescence analysis of HeLa cells using HSPD1 antibody. Blue: DAPI for nuclear staining.
HSPD1 / HSP60 Antibody - Western blot analysis of extracts of various cell lines, using HSPD1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
HSPD1 / HSP60 Antibody - Western blot analysis of extracts of various cell lines.
HSPD1 / HSP60 Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug HSPD1 antibody. Western blot was performed from the immunoprecipitate using HSPD1 antibodyat a dilition of 1:1000.
HSPD1 / HSP60 Antibody - Immunohistochemistry of paraffin-embedded rat kidney using HSPD1 antibodyat dilution of 1:100 (40x lens).
HSPD1 / HSP60 Antibody - Immunofluorescence analysis of HeLa cells using HSPD1 antibody. Blue: DAPI for nuclear staining.
HSPD1 / HSP60 Antibody - Western blot analysis of extracts of various cell lines, using HSPD1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 10s.
HSPD1 / HSP60 Antibody - Western blot analysis of extracts of various cell lines.
HSPD1 / HSP60 Antibody - Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug HSPD1 antibody. Western blot was performed from the immunoprecipitate using HSPD1 antibodyat a dilition of 1:1000.
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Polyclonal Rabbit anti‑Human HSPD1 / HSP60 Antibody (IHC, IF, WB) LS‑C331177

Polyclonal Rabbit anti‑Human HSPD1 / HSP60 Antibody (IHC, IF, WB) LS‑C331177

Antibody:
HSPD1 / HSP60 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$263
LS-C331177-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HSPD1 / HSP60 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HSP60 antibody LS-C331177 is an unconjugated rabbit polyclonal antibody to HSP60 (HSPD1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, IP and WB.
Target
Human HSPD1 / HSP60
Synonyms
HSPD1 | 60 kDa chaperonin | CPN60 | Heat shock protein 65 | Heat shock protein 60 | HSP-60 | HSP60 | HuCHA60 | HLD4 | HSP65 | p60 lymphocyte protein | SPG13 | Chaperonin 60 | GROEL
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 27-240 of human HSPD1 (NP_002147.2). AKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQ
Specificity
Human HSPD1 / HSP60
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:50 - 1:200)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 17kDa/61kDa, while the observed MW by Western blot was 61kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPD1 / HSP60
P10809 NM_002156 NP_002147.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/28/2022