This antibody detects an ~60kD protein, corresponding to the apparent molecular mass of Hsp60 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, dolphin, Drosophila, fish (rainbow trout), guinea pig, hamster, insect (mosquito), monkey, porcine, rabbit and snake. This antibody recognizes a surface epitope of Hsp60 in the region of amino acids 288-366 and does not cross react with bacterial or yeast Hsp60 counterparts.
Western Blot (Colorimetric): 1:10000. Immunoprecipitation. Immunohistochemistry. Immunocytochemistry. FACS. ELISA. Immunoelectron Microscopy. Blocking. Positive control: HeLa Heat Shocked Cell Lysate.
PBS, pH 7.2, 0.1 mM PMSF, 50% Glycerol
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
This antibody carries the LSBio 100% Guarantee.