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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2040-50 | 50 µg (0.5 mg/ml) | $460 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human HSPD1 / HSP60 Antibody (aa150‑200, IHC, WB) LS‑B2040
IHC‑plus™ Polyclonal Rabbit anti‑Human HSPD1 / HSP60 Antibody (aa150‑200, IHC, WB) LS‑B2040
Note: This antibody replaces LS-C19406
Antibody:
HSPD1 / HSP60 Rabbit anti-Human Polyclonal (aa150-200) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Bovine, Dog
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
HSPD1 / HSP60 Rabbit anti-Human Polyclonal (aa150-200) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Bovine, Dog
Format:
Unconjugated, Unmodified
Specifications
Description
HSP60 antibody LS-B2040 is an unconjugated rabbit polyclonal antibody to HSP60 (HSPD1) (aa150-200) from human. It is reactive with human, bovine, dog and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human HSPD1 / HSP60
Synonyms
HSPD1 | 60 kDa chaperonin | CPN60 | Heat shock protein 65 | Heat shock protein 60 | HSP-60 | HSP60 | HuCHA60 | HLD4 | HSP65 | p60 lymphocyte protein | SPG13 | Chaperonin 60 | GROEL
Host
Rabbit
Reactivity
Human, Monkey, Bovine, Dog
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
A portion of amino acids 150-200 of human Hsp60.
Epitope
aa150-200
Specificity
The amino acid sequence used as immunogen is 100% homologous in a broad range of species including human, dog, mouse, rat, bovine, chimp, monkey, and orangutan.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.5 - 2 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2040 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2040 was determined to be 5 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HSPD1 / HSP60
Validation
Anti-HSPD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-HSPD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ HSPD1 / HSP60 Antibody (aa150-200) for IHC, WB/Western LS-B2040 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
In vitro and in vivo studies of the ALS-FTLD protein CHCHD10 reveal novel mitochondrial topology and protein interactions. Burstein SR, Valsecchi F, Kawamata H, Bourens M, Zeng R, Zuberi A, Milner TA, Cloonan SM, Lutz C, Barrientos A, Manfredi G. Human molecular genetics. 2018 January;27:160-177.
Distinct Roles of Mitochondrial HIGD1A and HIGD2A in Respiratory Complex and Supercomplex Biogenesis. Alba Timón-Gómez, Joshua Garlich, Rosemary A Stuart, Cristina Ugalde, Antoni Barrientos. Cell reports. 2020 May;31:107607.
Customer Reviews (0)
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
