Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > HSPB2 / HSP27 > LS-C368882
 
Print Page  
Catalog Number Size Price
LS-C368882-30 30 µl (1 mg/ml) $255 
LS-C368882-100 100 µl (1 mg/ml) $299 
LS-C368882-200 200 µl (1 mg/ml) $351 
HSPB2 / HSP27 Antibody - Immunohistochemical analysis of HSPB2 staining in human renal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HSPB2 / HSP27 Antibody - Immunofluorescent analysis of HSPB2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HSPB2 / HSP27 Antibody - Western blot analysis of HSPB2 expression in HeLa (A); human heart (B) whole cell lysates.
HSPB2 / HSP27 Antibody - Immunohistochemical analysis of HSPB2 staining in human renal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HSPB2 / HSP27 Antibody - Immunofluorescent analysis of HSPB2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HSPB2 / HSP27 Antibody - Western blot analysis of HSPB2 expression in HeLa (A); human heart (B) whole cell lysates.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human HSPB2 / HSP27 Antibody (C‑Terminus, IHC, IF, WB) LS‑C368882

Polyclonal Rabbit anti‑Human HSPB2 / HSP27 Antibody (C‑Terminus, IHC, IF, WB) LS‑C368882

Submit Review LSBio Guarantee
Antibody:
HSPB2 / HSP27 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255
LS-C368882-30
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HSPB2 / HSP27 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
HSP27 antibody LS-C368882 is an unconjugated rabbit polyclonal antibody to HSP27 (HSPB2) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human HSPB2 / HSP27
Synonyms
HSPB2 | DMPK-binding protein | Heat shock protein beta-2 | HSP27 | Heat-shock protein beta-2 | Hs.78846 | MKBP | Heat shock 27kD protein 2 | Heat shock 27kDa protein 2 | LOH11CR1K
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human HSPB2.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of HSPB2 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPB2 / HSP27
Q16082 NM_001541 NP_001532.1

Publications (0)

Submit a Publication for LS-C368882

Customer Reviews (0)

Submit a review for LS-C368882

Featured Products

HSPB2 / HSP27 Antibody - Western blot of HSPB2 Antibody with human HeLa Whole Cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HSPB2 / HSP27 Antibody (N‑Terminus) LS‑C345460
Species: Human
Applications: Western blot
HSPB2 / HSP27 Antibody - Western blot of HSP beta2 antibody
HSPB2 / HSP27 Antibody (90‑170 aa, C‑terminal) LS‑C384027
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA
HSPB2 / HSP27 Antibody - IHC of paraffin-embedded breast cancer tissues (left) and cardiac muscle tissues (right) using HSP27 mouse monoclonal antibody with DAB staining.
HSPB2 / HSP27 Antibody (clone 5D7) LS‑C169363
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
ACTB / Beta Actin Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 5 ug/ml.
IHC‑plus™ ACTB / Beta Actin Antibody (N‑Terminus) LS‑B15553
Species: Human, Monkey, Mouse, Rat, Dog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
SFTPC / Surfactant Protein C ELISA Kit
Human SFTPC / Surfactant Protein C (Sandwich ELISA) ELISA Kit - LS-F12438
Reactivity: Human
Range: 0.469-30 ng/ml
Alpha Amylase ELISA Kit
Human Alpha Amylase (Sandwich ELISA) ELISA Kit - LS-F4946
Reactivity: Human
Range: 1.56-100 ng/ml
See all HSPB2 / HSP27 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com