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Catalog Number Size Price
LS-C368882-30 30 µl (1 mg/ml) $245 
LS-C368882-100 100 µl (1 mg/ml) $289 
LS-C368882-200 200 µl (1 mg/ml) $341 
HSPB2 / HSP27 Antibody - Immunohistochemical analysis of HSPB2 staining in human renal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HSPB2 / HSP27 Antibody - Immunofluorescent analysis of HSPB2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HSPB2 / HSP27 Antibody - Western blot analysis of HSPB2 expression in HeLa (A); human heart (B) whole cell lysates.
HSPB2 / HSP27 Antibody - Immunohistochemical analysis of HSPB2 staining in human renal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HSPB2 / HSP27 Antibody - Immunofluorescent analysis of HSPB2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
HSPB2 / HSP27 Antibody - Western blot analysis of HSPB2 expression in HeLa (A); human heart (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human HSPB2 / HSP27 Antibody (C‑Terminus, IHC, IF, WB) LS‑C368882

Polyclonal Rabbit anti‑Human HSPB2 / HSP27 Antibody (C‑Terminus, IHC, IF, WB) LS‑C368882

Antibody:
HSPB2 / HSP27 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$245
LS-C368882-30
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
HSPB2 / HSP27 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
HSP27 antibody LS-C368882 is an unconjugated rabbit polyclonal antibody to HSP27 (HSPB2) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human HSPB2 / HSP27
Synonyms
HSPB2 | DMPK-binding protein | Heat shock protein beta-2 | HSP27 | Heat-shock protein beta-2 | Hs.78846 | MKBP | Heat shock 27kD protein 2 | Heat shock 27kDa protein 2 | LOH11CR1K
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human HSPB2.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of HSPB2 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPB2 / HSP27
Q16082 NM_001541 NP_001532.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/1/2021