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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C15719-50 50 µg $425 
LS-C15719-200 200 µg $680 
Antibody

Polyclonal Rabbit anti‑Human HSPB1 / HSP27 Antibody (IHC, WB) LS‑C15719

Polyclonal Rabbit anti‑Human HSPB1 / HSP27 Antibody (IHC, WB) LS‑C15719

Antibody:
Rabbit Polyclonal to Human HSPB1 / HSP27
Application:
IHC, ICC, WB, IP, ELISA
Reactivity:
Human, Monkey, Guinea pig, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C15719-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human HSPB1 / HSP27
Application:
IHC, ICC, WB, IP, ELISA
Reactivity:
Human, Monkey, Guinea pig, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
HSP27 antibody LS-C15719 is an unconjugated rabbit polyclonal antibody to HSP27 (HSPB1) from human. It is reactive with human, guinea pig, monkey and other species. Validated for ELISA, ICC, IHC, IP and WB.
Target
Human HSPB1 / HSP27
Synonyms
HSPB1 | 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
Host
Rabbit
Reactivity
Human, Monkey, Guinea pig, Pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC (1:200)
  • ICC (1:300)
  • Western blot
  • Immunoprecipitation (1:100)
  • ELISA (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Hsp27 produced through recombinant DNA methods in E. coli. The purification of Hsp27 closely follows the method described in previously published reports.
Specificity
Detects a ~27kD protein, corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots, in samples from human, monkey, guinea pig and porcine (weakly).
Usage
Western Blot (Colorimetric): 1:5000. Immunoprecipitation: 1:100. Immunocytochemistry: 1:300. Immunohistochemistry: 1:200. ELISA: 1:1000. Positive control: Recombinant Human Hsp27 Protein.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPB1 / HSP27
P04792 NM_001540 NP_001531.1

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Requested From: United States
Date Requested: 7/4/2020