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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C15658-100 100 µg $755 
Antibody

Monoclonal Mouse anti‑Human HSPB1 / HSP27 Antibody (IHC, WB) LS‑C15658

Monoclonal Mouse anti‑Human HSPB1 / HSP27 Antibody (IHC, WB) LS‑C15658

Antibody:
Mouse Monoclonal to Human HSPB1 / HSP27
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$755 
LS-C15658-100
100 µg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human HSPB1 / HSP27
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HSP27 antibody LS-C15658 is an unconjugated mouse monoclonal antibody to human HSP27 (HSPB1). Validated for ELISA, ICC, IHC, IP and WB.
Target
Human HSPB1 / HSP27
Synonyms
HSPB1 | 28 kDa heat shock protein | Heat shock 27 kDa protein | Heat shock 27kD protein 1 | Heat shock 27kDa protein 1 | HSP27 | HSP28 | HSP 27 | Hsp25 | SRP27 | Stress-responsive protein 27 | CMT2F | Heat shock 27 kd protein | Heat shock protein beta-1 | HMN2B | HS.76067
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • ICC
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Prokaryotic recombinant protein corresponding to the full length human hsp27 protein.
Specificity
Recognizes HSP27 in human tissues and cell lines at 27 kD. Does not cross react with HSP70, HSP90 or HSP104.
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:500-1:1000. Immunohistochemistry (Frozen and paraffin): 1:50. Immunocytochemistry: Frozen and paraffin. Positive control: MCF-7 cells.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide, No stabilizing proteins added
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPB1 / HSP27
P04792 NM_001540 NP_001531.1

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Requested From: United States
Date Requested: 7/4/2020